Marie Curie International Outgoing Fellowship (European Union's FP/2007-2013 Grant Agreements #221834 and 254780), J.D. McDonnell Foundation, NSF Grant SBE 0542013, National Institutes of Health Grant NS034994, National Institute of Mental Health Grant MH5467 and the Howard Hughes Medical Institute (Janelia Farm Research Campus grant).

1. Construction of the microdrive
All drives are made from the same basic elements: a moving part, which carries the electrode and a fixed part, which is anchored to the skull. An ideal microdrive allows smooth but long enough travel of the electrode in multiple small steps, is sturdy enough to prevent accidental movement of the electrode, easy to manipulate by the experimenter without interfering with the animal's behavior, small in size and light in weight. As a result of these competing requi...

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This movie illustrates the implantation procedure of silicon probes for chronic large-scale recordings in the behaving rat. Critical steps to ensure quality recordings of neuronal activity arise from the fragility of both biological (brain tissue) and technical (silicon probe) materials. Special care should be taken while handling the probe to avoid any contact of shanks with any remotely "hard" surface (for example, the shanks would break if one tried to implant them in the brain without removing the dura). Similarly, a...

<table border="1">
	<tbody>
		<tr>
			<td>Name</td>
			<td>Type</td>
			<td>Company</td>
			<td>Catalog Number</td>
			<td>Comments</td>
		</tr>
		<tr>
			<td>Silicon probe Buzsaki32, 4 shanks x 8 sites. Packaging: flexible polyamide cable</td>
			<td>Material</td>
			<td>NeuroNexus</td>
			<td>Probe: buzsaki32 Packaging: HC32</td>
			<td>Recording probe</td>
		</tr>
		<tr>
			<td>Round Brass Screw, 00-90 x 1/2 Round Brass Screws</td>
			<td>Material</td>
			<td>JIMorris</td>
			<td>R0090B500</td>
			<td>Drive part</td>
		</tr>
		<tr>
			<td>Brass Hex Nut, 00-90</td>
			<td>Material</td>
			<td>JIMorris</td>
			<td>N0090B</td>
			<td>Drive part</td>
		</tr>
		<tr>
			<td>Brass C260 Strip, ASTM-B36 
			Thickness: 0.025", Length: 12", Width: 1/2"</td>
			<td>Material</td>
			<td>Small Parts</td>
			<td>B000FMYU72</td>
			<td>Drive part</td>
		</tr>
		<tr>
			<td>Connector Header, pitch 2mm, male, single row, straigt, 36 positions</td>
			<td>Material</td>
			<td>Digikey</td>
			<td>2163S-36-ND</td>
			<td>Drive part</td>
		</tr>
		<tr>
			<td>2-part Sylgard silicon Elastomer</td>
			<td>Material</td>
			<td>World Precision Instruments</td>
			<td>SYLG184</td>
			<td>To extra-insulate the probe</td>
		</tr>
		<tr>
			<td>Decon Contrad 70 Liquid Detergent</td>
			<td>Reagent</td>
			<td>Fisher Scientific</td>
			<td>04-355 
			Decon Laboratories 
			No.:1002</td>
			<td>To clean the recording sites</td>
		</tr>
		<tr>
			<td>Impedance Conditioning Module</td>
			<td>Equipment</td>
			<td>FHC Inc.</td>
			<td>55-70-0</td>
			<td>Impedance meter</td>
		</tr>
		<tr>
			<td>niPOD &#x2013; 32 channels</td>
			<td>Equipment</td>
			<td>Neuronexus</td>
			<td>niPOD -32</td>
			<td>Impedance meter</td>
		</tr>
		<tr>
			<td>Grip Cement Industrial Grade</td>
			<td>Material</td>
			<td>Caulk Dentsply</td>
			<td>675571 (powder) 
			675572 (solvent)</td>
			<td>Grip cement</td>
		</tr>
		<tr>
			<td>1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate ('DiI'; DiIC18(3))</td>
			<td>Reagent</td>
			<td>Invitrogen</td>
			<td>D282</td>
			<td>To stain the probe track in the brain</td>
		</tr>
		<tr>
			<td>Stainless Steel Machine Screw, Binding Head, Slotted Drive, #00-90, 1/8" </td>
			<td>Material</td>
			<td>Small Parts</td>
			<td>MX-0090-02B</td>
			<td>Ground and reference screws</td>
		</tr>
		<tr>
			<td>Magnet wire, 20G, nylon-polyurethane coating, MW80</td>
			<td>Material</td>
			<td>Small Parts</td>
			<td>B000IJYRP2</td>
			<td>Ground and reference wire</td>
		</tr>
		<tr>
			<td>Stainless Steel Machine Screw, Binding Head Slotted Drive, #000-120, 1/16"</td>
			<td>Material</td>
			<td>Small Parts</td>
			<td>MX-000120-01B</td>
			<td>Anchor screws</td>
		</tr>
		<tr>
			<td>N-3 All purpose Flux Liquid</td>
			<td>Reagent</td>
			<td>La-Co (Markal)</td>
			<td>23512</td>
			<td>Allows to solder stainless-steel</td>
		</tr>
		<tr>
			<td>MicroGrid Precision Expanded Copper</td>
			<td>Material</td>
			<td>Dexmet</td>
			<td>3 CU6-050 FA</td>
			<td>Copper mesh for on-head Faraday cage</td>
		</tr>
		<tr>
			<td>C&amp;B-METABOND Quick! Cement System - Dentin Activator</td>
			<td>Material</td>
			<td>Parkell</td>
			<td>S380</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>C&amp;B-METABOND Quick! Cement System &#x2013; Dental cement</td>
			<td>Material</td>
			<td>Parkell</td>
			<td>S380</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Sharp point tungsten needle and holder</td>
			<td>Tool</td>
			<td>Roboz Surgical instruments</td>
			<td>RS-6064 and RS-6061</td>
			<td>To make the hook to lift the dura</td>
		</tr>
		<tr>
			<td>Carbide Bur HP 1/4</td>
			<td>Tool</td>
			<td>Henry Schein</td>
			<td>9990013</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Paraffin (Granules)</td>
			<td>Material</td>
			<td>Fisher Scientific</td>
			<td>P31-500</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Mineral Oil, Light (NF/FCC)</td>
			<td>Material</td>
			<td>Fisher Scientific</td>
			<td>O121-1</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>GC ELECTRONICS 10-114 2-Part Epoxy Adhesive</td>
			<td>Material</td>
			<td>Newark</td>
			<td>00Z416</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Type 1 LITZ 21 AWG 40/36 Red Single Polyurethane-Nylon (MW80-C) TO 0.041"+/-0.002" OD</td>
			<td>Material</td>
			<td>New England Wire Technologies Corporation</td>
			<td>N28-36E-400-2</td>
			<td>To make the cable between the headstage and the amplifier</td>
		</tr>
		<tr>
			<td>32-channel Very Large Scale Integration headstage, 20x gain</td>
			<td>Equipment</td>
			<td>Plexon</td>
			<td>HST/32V-G20</td>
			<td>Headstage</td>
		</tr>
	</tbody>
</table>

large-scale recording of neurons by movable silicon probes in behaving rodents

A major challenge in neuroscience is linking behavior to the collective activity of neural assemblies. Understanding of input-output relationships of neurons and circuits requires methods with the spatial selectivity and temporal resolution appropriate for mechanistic analysis of neural ensembles in the behaving animal, i.e. recording of representatively large samples of isolated single neurons. Ensemble monitoring of neuronal activity has progressed remarkably in the past decade in both small and large-brained animals, including human subjects1-11. Multiple-site recording with silicon-based devices are particularly effective because of their scalability, small volume and geometric design.
Here, we describe methods for recording multiple single neurons and local field potential in behaving rodents, using commercially available micro-machined silicon probes with custom-made accessory components. There are two basic options for interfacing silicon probes to preamplifiers: printed circuit boards and flexible cables. Probe supplying companies (<a href="http://www.neuronexustech.com/" >http://www.neuronexustech.com/</a>; <a href="http://www.sbmicrosystems.com/">http://www.sbmicrosystems.com/</a>; <a href="http://www.acreo.se/">http://www.acreo.se/</a>) usually provide the bonding service and deliver probes bonded to printed circuit boards or flexible cables. Here, we describe the implantation of a 4-shank, 32-site probe attached to flexible polyimide cable, and mounted on a movable microdrive. Each step of the probe preparation, microdrive construction and surgery is illustrated so that the end user can easily replicate the process.

Watch this Scientific Journal Video about Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents at JoVE.com

Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents

We describe methods for large-scale recording of multiple single units and local field potential in behaving rodents with silicon probes. Drive fabrication, probe attachment to the drive and probe implantation processes are illustrated in sufficient details for easy replication.

A major challenge in neuroscience is linking behavior to the collective activity of neural assemblies. Understanding of input-output relationships of ...

large-scale-recording-neurons-movable-silicon-probes-behaving

Research

JoVE Journal

Neuroscience

34.4K visualizações.  University of New Jersey. Descrevemos os métodos de larga escala de gravação de várias unidades e potencial de campo local, comportando roedores com sondas de silício. Unidade de fabricação, o apego sonda para a unidade e os processos de implantação de teste são ilustrados em detalhes suficientes para a replicação fácil.