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JoVE Journal

Immunology and Infection

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Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine

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09:25 min

May 21st, 2012

May 21st, 2012

38,833 Views

0:05

Title

1:15

Dissection and Dissociation of Intestinal Epithelial Cells

3:56

Tissue Digestion and Isolation of Intestinal Cells

5:47

Gating Strategy for Laimna Propria Antigen-presenting Cells

7:55

Results: Cell Yield and Antibody Staining Quality Depends on Digestion Time

8:58

Conclusion

Here, we detail a methodology for the rapid isolation of mouse intestinal dendritic cells (DCs) and macrophages. Phenotypic characterization of intestinal DCs and macrophages is performed using multi-color flow cytometric analysis while magnetic bead enrichment followed by cell sorting is used to yield highly pure populations for functional studies.

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