Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine

39.0K Views

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09:25 min

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May 21st, 2012

DOI :

10.3791/4040-v

May 21st, 2012

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Duke Geem¹, Oscar Medina-Contreras¹, Wooki Kim², Clifton S. Huang¹, Timothy L. Denning¹^,²

¹Department of Pediatrics, Emory University, ²Department of Pathology & Laboratory Medicine, Emory University

Summary

Chapters in this video

0:05

Title

1:15

Dissection and Dissociation of Intestinal Epithelial Cells

3:56

Tissue Digestion and Isolation of Intestinal Cells

5:47

Gating Strategy for Laimna Propria Antigen-presenting Cells

7:55

Results: Cell Yield and Antibody Staining Quality Depends on Digestion Time

8:58

Conclusion

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