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JoVE Journal

Chemistry

This content is Open Access.

Profiling the Triacylglyceride Contents in Bat Integumentary Lipids by Preparative Thin Layer Chromatography and MALDI-TOF Mass Spectrometry

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09:18 min

September 5th, 2013

September 5th, 2013

21,005 Views

0:05

Title

1:26

Tissue Treatment and Lipid Extraction

2:33

Lipid Separation by Preparative Thin-layer Chromatography

5:34

TAG Analysis by MALDI-TOF MS

7:21

Results: Triacylglyceride Profiles of Eastern Red Bat Tissues

8:30

Conclusion

Mammalian integument contains solvent-extractable lipids that can provide chemical compositions characteristic of individual species. This paper presents a routine method for separating broad lipid classes isolated from integumentary tissues using thin layer chromatography and determining the triacylglyceride profile by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

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