March 1st, 2014
•Serum utilized in embryo cultures contains unknown components that can affect the outcome of experiments especially in studies involving signaling interactions. Here we utilized a serum-free oxygenated culture system and show that mid-gestation mouse embryos cultured for 16-40 hr exhibit morphological development comparable to embryos developing in utero.
Tags
Vídeos Relacionados
Culture and Maintenance of Human Embryonic Stem Cells
Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching
An ex-ovo Chicken Embryo Culture System Suitable for Imaging and Microsurgery Applications
The Method of Rodent Whole Embryo Culture using the Rotator-type Bottle Culture System
Closed System Cell Culture Protocol Using HYPERStack Vessels with Gas Permeable Material Technology
Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo
A System for ex vivo Culturing of Embryonic Pancreas
A Novel Ex vivo Culture Method for the Embryonic Mouse Heart
An Ex vivo Culture System to Study Thyroid Development
Mouse Fetal Whole Intestine Culture System for Ex Vivo Manipulation of Signaling Pathways and Three-dimensional Live Imaging of Villus Development
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados