May 27th, 2016
•With the murine ABC transporter Bcrp1 (Abcg2) as an example, in-silico protocols are presented to detect alternative promoter usage in genes expressed in mouse tissues, and to evaluate the functionality of the alternative promoters identified using reporter assays.
Tags
Vídeos Relacionados
Dissection and Staining of Drosophila Larval Ovaries
Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes
Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA
Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides
A Quantitative Assay to Study Protein:DNA Interactions, Discover Transcriptional Regulators of Gene Expression, and Identify Novel Anti-tumor Agents
Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases In Vivo
Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly
Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules
Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean
Plant-Microbe Interaction: Transcriptional Response of Bacillus Mycoides to Potato Root Exudates
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados