Construction of the Disruption Cassette for Deleting KU70
3:08
Generation of the Y. lipolytica KU70 Deletion Strain
5:45
Cre Recombinase-mediated Marker Rescue
7:59
Results: Deletion of KU70 Enhances Gene Deletion Efficiency
9:27
Conclusion
Transcrição
The overall goal of this procedure is to develop effective genetic engineering methods for an unconventional yeast species, including construction of transforming DNA, DNA transformation, transformant selection, and targeted gene deletion by homol
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We herein report methods on the molecular genetic manipulation of the Yarrowia lipolytica Po1g strain for improved gene deletion efficiency. The resulting engineered Y. lipolytica strains have potential applications in biofuel and biochemical production.