April 16th, 2018
•Here we present a protocol for cardiac-specific gene manipulation in mice. Under anesthesia, the mouse hearts were externalized through the fourth intercostal space. Subsequently, adenoviruses encoding specific genes were injected with a syringe into the myocardium, followed by protein expression measurement via in vivo imaging and Western blot analysis.
Vídeos Relacionados
Pyrosequencing: A Simple Method for Accurate Genotyping
Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA
Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides
Simple and Efficient Technique for the Preparation of Testicular Cell Suspensions
In Vivo SiRNA Transfection and Gene Knockdown in Spinal Cord via Rapid Noninvasive Lumbar Intrathecal Injections in Mice
In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow
Intravenous Injections in Neonatal Mice
Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method
A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
Simple Detection of Primary Cilia by Immunofluorescence
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados