DC Purification by Positive Magnetic Bead Selection
8:02
Allogeneic Stimulation Assay to Assess DC Cell Function
10:19
Results: Successful Isolation of DCs from the Human Female Reproductive Tract
11:55
Conclusion
Transcrição
The overall goal of this procedure is to isolate dendritic cells from different anatomical compartments in the human female reproductive tract to evaluate their phenotypical and functional characteristics. This method can help answer key questions
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We describe here a method to isolate and purify dendritic cells from different anatomical compartments in the human female reproductive tract for the evaluation of their phenotypical and functional characteristics. This method can be adapted to isolate other immune cells or dendritic cells from other mucosal tissues.