Hi, my name is Dr.Robert Schaut and I'm a scientist at the National Animal Disease Center in Ames, Iowa. In this JoVE video, we will be sharing with you information about the collection and processing of lymph nodes from large animals for use in d
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This protocol provides an overview of procedures for the isolation of RNA for the transcriptomic profiling of lymph node tissues from large animals, including steps in the identification and excision of lymph nodes from livestock and wildlife, sampling approaches to provide consistency across multiple animals, and considerations plus representative results for the post-collection preservation and processing for RNA analysis.