Human Amniotic Membrane (hAM) Thermolysin Treatment and Fixation
2:27
Retinal Pigment Epithelium (RPE) Cell Seeding
3:33
RPE Patch Transplantation Preparation
5:58
Results: Representative RPE-seeded hAM
6:38
Conclusion
Transcrição
This method can help answer key questions about the self therapy of the eye, such as what is the efficacy of a specifically designed bio-engineered tissue in vivo. With this technique we provide a method of producing and contouring the retinal tis
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We describe a method to engineer a retinal tissue composed of retinal pigment epithelial cells derived from human pluripotent stem cells cultured on top of human amniotic membranes and its preparation for grafting in animal models.