Engineering Transplantation-suitable Retinal Pigment Epithelium Tissue Derived from Human Embryonic Stem Cells

7.6K Views

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07:48 min

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September 6th, 2018

DOI :

10.3791/58216-v

September 6th, 2018

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Karim Ben M'Barek¹^,²^,³, Walter Habeler¹^,²^,³, Alexandra Plancheron¹^,²^,³, Mohamed Jarraya⁴, Olivier Goureau⁵, Christelle Monville¹^,²

¹U861, I-Stem, Association Française contre les Myopathies (AFM), Institut National de la Santé et de la Recherche Médicale (INSERM), ²U861, I-Stem, Association Française contre les Myopathies (AFM), Université Evry Val-d'Essonne (UEVE), ³I-Stem, Association Française contre les Myopathies (AFM), Centre pour L’Etude des Cellules Souches (CECS), ⁴Banque de tissus humain, Hôpital Saint Louis, Assistance Publique - Hôpitaux de Paris (AP-HP), ⁵Sorbonne Université, INSERM, CNRS, Institut de la Vision, F-75012

Summary

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Keywords Retinal Pigment Epithelium

Human Embryonic Stem Cells

Chapters in this video

0:04

Title

0:36

Human Amniotic Membrane (hAM) Thermolysin Treatment and Fixation

2:27

Retinal Pigment Epithelium (RPE) Cell Seeding

3:33

RPE Patch Transplantation Preparation

5:58

Results: Representative RPE-seeded hAM

6:38

Conclusion

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