JoVE Logo
Centro de Recursos para Docentes

Entrar

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

DOI :

10.3791/58244-v

September 13th, 2018

September 13th, 2018

6,664 Views

1Department of Microbiology, University of Texas Southwestern Medical Center

Here, we present the protocols to identify 1) virus-encoded immunomodulators that promote arbovirus replication and 2) eukaryotic host factors that restrict arbovirus replication. These fluorescence- and luminescence-based methods allow researchers to rapidly obtain quantitative readouts of arbovirus replication in simplistic assays with low signal-to-noise ratios.

Tags

Arbovirus Infections

-- Visualizações

Vídeos Relacionados

article

RNAi Screening for Host Factors Involved in Vaccinia Virus Infection using Drosophila Cells

article

Introducing Shear Stress in the Study of Bacterial Adhesion

article

Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro

article

Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis

article

VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance

article

Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants

article

Early Viral Entry Assays for the Identification and Evaluation of Antiviral Compounds

article

The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii

article

Human Placental and Decidual Organ Cultures to Study Infections at the Maternal-fetal Interface

article

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

JoVE Logo

Privacidade

Termos de uso

Políticas

Pesquisa

Educação

SOBRE A JoVE

Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados