Preparation of Sample Block Containing Cancer Spheroids for Frozen Tissue Sectioning
5:36
Immunofluorescence Imaging for Frozen Sectioned Cancer Spheroids
7:18
Results: Invasive Bladder Cancer Tumor Spheroids Formed from Cell Lines and Primary Tumors
8:45
Conclusion
TRANSCRIÇÃO
Invasive progression is a crucial determinant of clinical outcome in patients with bladder cancer. Here we will describe a simple in vitro method, which utilizes tumor spheroids generated using cell lines or primary tumors to study invasion. The m
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The processes governing bladder cancer invasion represent opportunities for biomarker and therapeutic development. Here we present a bladder cancer invasion model which incorporates 3-D culture of tumor spheroids, time-lapse imaging and confocal microscopy. This technique is useful for defining the features of the invasive process and for screening therapeutic agents.