Results: Capturing and Analyzing PKR-interacting RNAs During the Cell Cycle Using qRT-PCR
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Conclusion
TRANSCRIÇÃO
This protocol provides a method to identify RNA interactors of an RNA binding protein PKR in a genome-wide manner. It helps us to better understand post-transcriptional regulation of gene expression by RNA binding protein. The main advantage of th
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We present experimental approaches for studying RNA-interactors of double-stranded RNA binding protein kinase RNA-activated (PKR) during the mammalian cell cycle using HeLa cells. This method utilizes formaldehyde to crosslink RNA-PKR complexes and immunoprecipitation to enrich PKR-bound RNAs. These RNAs can be further analyzed through high-throughput sequencing or qRT-PCR.