Results: Synthesized LIMK2-1 Protein, Its Interaction with Kinase ROCK, its Kinase Activity
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Conclusion
TRANSCRIÇÃO
Immunoprecipitation is a very powerful technique to isolate and purify a target protein. In smooth conditions, proteins, regulators, or substrates may be co-immunoprecipitated. A new interaction network may then be discovered.
The activity o
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We characterized a new kinase protein using robust biochemical approaches: Western Blot analysis with a dedicated specific antibody on different cell lines and tissues, interactions by coimmunoprecipitation experiments, kinase activity detected by Western Blot using a phospho-specific antibody and by γ[32P] ATP labeling.