Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

DOI :

10.3791/60740-v

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11:00 min

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March 25th, 2020

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16,305 Views

Manuel Flores Molina^1,2, Thomas Fabre^1,2, Aurélie Cleret-Buhot¹, Geneviève Soucy^1,3, Liliane Meunier^1,4, Mohamed N. Abdelnabi^1,2, Nicolas Belforte^1,5, Simon Turcotte^1,4,6, Naglaa H. Shoukry^1,4,7

¹Centre de Recherche du Centre hospitalier de l'Université de Montréal (CRCHUM), ²Département de Microbiologie, Infectiologie et Immunologie, Faculté de Médecine, Université de Montréal, ³Département de Pathologie et Biologie Cellulaire, Faculté de Médecine, Université de Montréal, ⁴Institut du Cancer de Montréal, ⁵Département de Neurosciences, Faculté de Médecine, Université de Montréal, ⁶Département de Chirurgie, Faculté de Médecine, Université de Montréal, ⁷Département de Médecine, Faculté de médecine, Université de Montréal

Chapters

Here, we describe a simple and accessible strategy for visualizing, quantifying, and mapping immune cells in formalin-fixed paraffin-embedded tumor tissue sections. This methodology combines existing imaging and digital analysis techniques with the purpose of expanding the multiplexing capability and multiparameter analysis of imaging assays.

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