Este trabalho foi apoiado por um Grant-in-Aid for Scientific Research C (grant no. 19K09834 to K.N.) do Ministério da Educação, Ciência e Cultura, Japão.

Todos os procedimentos foram realizados sob o protocolo aprovado pelo Comitê de Ética para o bem-estar humano. Todos os pacientes forneceram consentimento informado por escrito para o uso em pesquisa de suas amostras, e a coleta e o uso de tecidos para este estudo foram aprovados pelo Comitê de Ética em Pesquisa em Análise de Genes do Genoma Humano, da Universidade de Teikyo.
1. Isolamento e cultura de células-tronco de câncer de ovário (OCSCs) de pacientes com câncer de ovário e ascite em um gabinete de biossegurança de nível 2
<ol><li>Isole células-tronco cancerígenas da ascite do câncer de ovário humano obtido por paracentese. Colete pelo menos 100-250 mL de ascite de pacientes para tomar um número suficiente de células-tronco cancerígenas. Além disso, avalie os perfis de expressão de marcadores de células-tronco cancerígenas (ou seja, EpCAM, Calretinina, CD133, CD44, CD45, ALDH1 e Oct4) e marcadores de câncer de ovário (pAX-8, WT-1) por citometria de fluxo.<ol><li>Centrifugar a ascite do cancro do ovário humano a 300 x g durante 10 minutos à temperatura ambiente no prazo de 24 h após a aspiração da ascite.</li>
		<li>Remover o sobrenadante e adicionar 2 mL de meio OCSC e 8 mL de solução salina tamponada com Histodenz/fosfato a 30% (PBS, pH 7,4).</li>
		<li>Prepare o meio OCSC: suplemento StemPro hESC, DMEM⁄F-12 com L-glutamina (meio GlutaMAX), 25% BSA, 100 μM 2-mercaptoetanol, 8 ng/mL FGF BASIC, 10 μM de insulina e 20 μM Y-27632.</li>
		<li>Sobrepor cuidadosamente 2 ml de meio OCSC à solução celular na fase 1.1.2 num tubo de 15 ml e centrífuga a 450 x g durante 20 minutos à temperatura ambiente num rotor de balde oscilante sem travagem.</li>
		<li>Transfira cuidadosamente a camada OCSC (sem ser perturbada na interfase) para um novo tubo de 15 mL por pipeta de transferência.</li>
		<li>Encha com PBS até 15 mL. Centrifugar a 300 x g durante 5 min à temperatura ambiente e remover o sobrenadante.</li>
		<li>Ressuspender o pellet celular em meio OCSC e semear em um prato de cultura de fixação ultrabaixa; as culturas devem ser mantidas a 37 °C em 5% de CO2.</li>
		<li>Mude o meio a cada três dias. Posicione cuidadosamente o prato de cultura por cerca de 1 minuto, descarte parte do sobrenadante e adicione o novo meio.</li>
	</ol></li>
	<li>Passagem de CSCs<ol><li>Recolher OCSCs num tubo de 15 ml e centrifugar a 200 x g durante 5 min à temperatura ambiente.</li>
		<li>Remova o sobrenadante, encha com PBS e centrifugar a 200 x g por 5 min à temperatura ambiente.</li>
		<li>Remova o sobrenadante, adicione 1 mL da solução de descolamento celular que consiste em enzimas proteolíticas e colagenolíticas (por exemplo, AccuMax) e incube a 37 °C por 10 min.</li>
		<li>Misturar bem por pipetagem e incubar a 37 °C durante 5 min. Certifique-se de que as células estão em uma única suspensão.</li>
		<li>Misture bem por pipetagem, encha com PBS e centrifuga a 300 x g por 5 min à temperatura ambiente.</li>
		<li>Remover o sobrenadante e ressuspender o pellet celular em meio OCSC para posterior semeadura em placas de cultura de fixação ultrabaixa e manutenção a 37 °C em CO2 a 5%.</li>
	</ol></li>
</ol>2. Cultura de células endoteliais HUEhT-1
<ol><li>Passagem de células HUEhT-1<ol><li>Retire o meio da placa de cultura HUEhT-1 e lave as células com PBS.</li>
		<li>Adicionar 1 mL de tripsina a 0,025% e incubar por 3 min à temperatura ambiente.</li>
		<li>Adicionar 5 mL de Meio de Crescimento Celular Endotelial 2, coletar células em um tubo de 15 mL e centrifugar a 200 x g por 5 min à temperatura ambiente.</li>
		<li>Retirar o sobrenadante, ressuspender o pellet celular em meio HUEhT-1 e semear as células em placas de cultura revestidas de colágeno, seguidas de manutenção a 37 °C em 5% de CO2.</li>
		<li>Mude o meio a cada três dias.</li>
	</ol></li>
</ol>3. Preparação da Placa de Cocultura NICO-1 para ensaio de formação de tubos utilizando células HUEhT-1
<ol><li>Monte NICO-1 e revestimento com o hidrogel à base de matriz extracelular (Matrigel Matrix).<ol><li>Monte um lado do NICO-1 seguindo as instruções do fabricante e mantenha-o no gelo.</li>
		<li>Cubra a superfície do NICO-1 com 300 μL de PBS frio e, em seguida, remova o tampão tamponamento.</li>
		<li>Adicionar 300 μL de hidrogel à base de matriz extracelular refrigerado e incubar a 37 °C durante 60 min.</li>
		<li>Para equilibrar, mergulhe o filtro com 100% de etanol e, em seguida, lave um filtro ICCP de 13 mm (0,6 μm) com PBS por 1 min.</li>
		<li>Monte o NICO-1 incluindo as partes principais do corpo A (câmara direita) e B (câmara esquerda), juntamente com o anel O e o filtro equilibrado.</li>
	</ol></li>
</ol>4. Semeando células HUEhT-1 e CSCs no sistema NICO-1
<ol><li>Preparar suspensões celulares HUEhT-1 tripsinizando as monocamadas celulares e ressuspendendo as células em meio de crescimento celular endotelial com soro fetal de bezerro a 2%.</li>
	<li>Adicione 1,2 mL da suspensão celular (1,5 x 105 células) a cada poço revestido de hidrogel à base de matriz extracelular.</li>
	<li>Adicione 1,5 mL de OCSCs cultivados por cinco dias ao outro poço.</li>
	<li>Incubar NICO-1 a 37 °C em 5% de CO2; a formação de tubos pode ser observada ao microscópio e a formação de redes em hidrogel extracelular à base de matriz, medida por meio do número de ramos.</li>
</ol>

<ol>
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Os autores não têm nada a revelar.

O protocolo apresentado descreve como imitar o microambiente tumoral de OCSCs em um ambiente in vitro. O componente primário do método constitui o modelo de cocultura altamente reprodutível obtido usando o sistema NICO-1, um sistema de co-cultura Transwell indireto. Muitos dos modelos de cocultura atualmente disponíveis examinam os efeitos do contato direto célula-célula em populações celulares cocultivadas 12,13,14,15,16,17,18.<sup class="xref...

O câncer de ovário é a oitava neoplasia maligna mais comum em mulheres em todo o mundo, com aproximadamente 300.000 novos diagnósticos e uma estimativa de 180.000 mortes anuais1. No diagnóstico inicial, o câncer de ovário geralmente se apresenta com sintomas graves, com cerca de 75% das pacientes já no estágio III-IV. Assim, a taxa de sobrevida em 5 anos é de <30% e a taxa de mortalidade é a mais alta entre os cânceres ginecológicos2, com a eficiência do tratamento para o câncer de ovário sendo altamente dependente de fatores clínicos, como a realização bem-sucedida da cirurgia de debulking, resistência à quimioterapia e recorrência após a terapia inicial.
Os tecidos do câncer de ovário são hierarquicamente organizados, com nem todos os componentes do tumor sendo igualmente capazes de gerar descendentes. As únicas células capazes de se auto-renovar e produzir uma população heterogênea de células tumorais são consideradas como representando células-tronco cancerígenas (CSCs)3. A auto-renovação do CSC e o início do tumor são acompanhados pela promoção da angiogênese para remodelar seu microambiente tumoral com a finalidade de manter um nicho de suporte. No entanto, modelos anteriores não puderam ser utilizados para análises in vitro devido à reprodutibilidade limitada do cultivo de CSCs derivadas de amostras clínicas devido à ruptura de esferoides após múltiplas passagens. Mais recentemente, métodos experimentais para cultivar CSCs de pacientes têm sido desenvolvidos para diversas aplicações 4,5,6,7. Em particular, ao explorar a característica de crescimento das CSCs formando esferoides em placas de fixação ultrabaixa com meio livre de soro, as CSCs cultivadas são induzidas a expressar um marcador de superfície de células-tronco que não é expresso em células tumorais normais com potencial de diferenciação de múltiplas linhagens 8,9.
Dados recentes têm mostrado que a persistência de (O)CSCs ovarianos dormentes visualizados como disseminação no peritônio está associada à sua regeneração como tumores recorrentes10. A compreensão das características moleculares e biológicas dos OCSCs pode, portanto, permitir o direcionamento e a erradicação efetivos dessas células, resultando em potencial remissão tumoral. Em particular, pouco se sabe sobre as características mecanicistas celulares e moleculares dos papéis das CSCs na angiogênese11. Portanto, no presente protocolo, utilizamos OCSCs derivados de pacientes em um ambiente in vitro para investigar a propriedade angiogênica de células endoteliais usando o modelo de cocultura, que pode imitar o microambiente tumoral de CSCs e células endoteliais no local metastático no ambiente clínico. Em última análise, como a neovascularização constitui um processo crítico necessário para apoiar o crescimento e a metástase do tumor, uma melhor compreensão de seu mecanismo permitirá o desenvolvimento de uma nova terapia direcionada para OCSCs no local metastático.
Aqui, apresentamos um protocolo para visualizar a etapa de neovascularização em torno das CSCs de forma temporal. A vantagem do protocolo inclui permitir investigações totalmente reprodutíveis usando o sistema de cocultura 3D, NICO-1, permitindo assim a observação dos efeitos em pacientes da capacidade de iniciação de tumores derivados do OCSC durante a angiogênese de células endoteliais.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>0.025% Trypsin&#160;</td>
			<td>Thermo</td>
			<td>R001100</td>
			<td></td>
		</tr>
		<tr>
			<td>10 mL Pipet</td>
			<td>Thermo</td>
			<td>170356N</td>
			<td></td>
		</tr>
		<tr>
			<td>1250 &#181;L Pipet tip</td>
			<td>QSP</td>
			<td>T112XLRS-Q</td>
			<td></td>
		</tr>
		<tr>
			<td>15 mL tube</td>
			<td>Nunc</td>
			<td>339650</td>
			<td></td>
		</tr>
		<tr>
			<td>200 &#181;L Pipet tip</td>
			<td>QSP</td>
			<td>T110RS-NEW</td>
			<td></td>
		</tr>
		<tr>
			<td>2-Mercaptoethanol</td>
			<td>Thermo (Gibco)</td>
			<td>21985023</td>
			<td></td>
		</tr>
		<tr>
			<td>5 mL Pipet</td>
			<td>Thermo</td>
			<td>170366N</td>
			<td></td>
		</tr>
		<tr>
			<td>50 mL tube</td>
			<td>Corning</td>
			<td>430290</td>
			<td></td>
		</tr>
		<tr>
			<td>AccuMAX</td>
			<td>Innovative Cell Technologies</td>
			<td>AM105</td>
			<td></td>
		</tr>
		<tr>
			<td>BioCoatTM Collagen I 60mm Dish</td>
			<td>Corning</td>
			<td>356401</td>
			<td></td>
		</tr>
		<tr>
			<td>Centrifuge</td>
			<td>KUBOTA</td>
			<td>2800</td>
			<td></td>
		</tr>
		<tr>
			<td>Costar 6 Well Clear Flat Bottom Ultra Low Attachment Multiple Well Plates</td>
			<td>Corning</td>
			<td>3471</td>
			<td></td>
		</tr>
		<tr>
			<td>Endothelial Cell Growth Medium 2&#160;</td>
			<td>PromoCell</td>
			<td>C-22011&#160;</td>
			<td></td>
		</tr>
		<tr>
			<td>Ethanol</td>
			<td>WAKO</td>
			<td>057-00456</td>
			<td></td>
		</tr>
		<tr>
			<td>FGF-Basic</td>
			<td>Thermo (Gibco)</td>
			<td>PHG0021</td>
			<td></td>
		</tr>
		<tr>
			<td>Histodenz</td>
			<td>SIGMA</td>
			<td>D2158</td>
			<td></td>
		</tr>
		<tr>
			<td>HUEhT-1 cell</td>
			<td>JCRB Cell Bank</td>
			<td>JCRB1458</td>
			<td></td>
		</tr>
		<tr>
			<td>ICCP Filter 0.6 &#181;m</td>
			<td>Ginrei Lab.</td>
			<td>2525-06</td>
			<td></td>
		</tr>
		<tr>
			<td>Insulin, human</td>
			<td>SIGMA (Roche)</td>
			<td>11376497001</td>
			<td></td>
		</tr>
		<tr>
			<td>Luminometer</td>
			<td>PerkinElmer</td>
			<td>ARVO MX-flad</td>
			<td></td>
		</tr>
		<tr>
			<td>Matrigel Matrix</td>
			<td>Corning</td>
			<td>356234</td>
			<td></td>
		</tr>
		<tr>
			<td>Microscope</td>
			<td>Yokogawa</td>
			<td>CQ-1</td>
			<td></td>
		</tr>
		<tr>
			<td>NICO-1</td>
			<td>Ginrei Lab.</td>
			<td>2501-02</td>
			<td></td>
		</tr>
		<tr>
			<td>OptiPlate-96</td>
			<td>PerkinElmer</td>
			<td>6005290</td>
			<td></td>
		</tr>
		<tr>
			<td>P1000 Pipet</td>
			<td>Gilson</td>
			<td>F123602</td>
			<td></td>
		</tr>
		<tr>
			<td>P200 Pipet</td>
			<td>Gilson</td>
			<td>F123601</td>
			<td></td>
		</tr>
		<tr>
			<td>PBS</td>
			<td>Thermo (Gibco)</td>
			<td>14190-144</td>
			<td></td>
		</tr>
		<tr>
			<td>StemPro hESC SFM</td>
			<td>Thermo (Gibco)</td>
			<td>A1000701</td>
			<td></td>
		</tr>
		<tr>
			<td>Transfer Pipet</td>
			<td>FALCON</td>
			<td>357575</td>
			<td></td>
		</tr>
		<tr>
			<td>Y-27632</td>
			<td>WAKO</td>
			<td>253-00513</td>
			<td></td>
		</tr>
	</tbody>
</table>

evaluating the angiogenetic properties of ovarian cancer stem-like cells using the three-dimensional co-culture system, nico-1

As células-tronco cancerígenas (CSCs) residem em um nicho de suporte, constituindo um microambiente composto por células estromais adjacentes, vasos e matriz extracelular. A capacidade das CSCs de participar do desenvolvimento do endotélio constitui uma característica importante que contribui diretamente para a compreensão geral dos mecanismos de tumorigênese e metástase tumoral. O objetivo deste trabalho é estabelecer uma metodologia reprodutível para investigar a capacidade de iniciação tumoral de células-tronco de câncer de ovário (OCSCs). Neste trabalho, examinamos o mecanismo de neovascularização entre células endoteliais e OCSCs, juntamente com as alterações morfológicas das células endoteliais, usando o modelo de co-cultura in vitro NICO-1. Este protocolo permite a visualização da etapa de neovascularização em torno dos OCSCs de forma temporal. A técnica pode fornecer informações sobre as propriedades angiogenéticas dos OCSCs na metástase tumoral.

Foram coletados fluidos de ascite obtidos de pacientes com câncer de ovário avançado durante cirurgia ou paracentese com o objetivo de realizar uma cultura estável a longo prazo para esferoides. Aqui, apresentamos casos de uma cultura esferoide de longo prazo de CSCs ovarianos denominadas CSC1 e CSC2. Ambas as linhagens celulares carregam o mesmo diagnóstico e perfis histológicos. Os papéis mecanicistas dos OCSCs subjacentes à interação com as células endoteliais necessárias p...

Watch this Scientific Journal Video about Evaluating the Angiogenetic Properties of Ovarian Cancer Stem-Like Cells using the Three-Dimensional Co-Culture System, NICO-1 at JoVE.com

Evaluating the Angiogenetic Properties of Ovarian Cancer Stem-Like Cells using the Three-Dimensional Co-Culture System, NICO-1

As células-tronco do câncer de ovário (OCSC) são responsáveis pelo início, recorrência, resistência terapêutica e metástase do câncer. Considera-se que o nicho vascular do OCSC promove a auto-renovação dos OCSCs, levando à quimiorresistência. Este protocolo fornece a base para o estabelecimento de um modelo de nicho vascular OCSC reprodutível in vitro.

Avaliando as propriedades angiogenéticas de células-tronco do câncer de ovário usando o Sistema de Cocultura Tridimensional, NICO-1

Cancer stem cells (CSCs) reside in a supportive niche, constituting a microenvironment comprised of adjacent stromal cells, vessels, and extracellular ...

evaluating-angiogenetic-properties-ovarian-cancer-stem-like-cells

Research

JoVE Journal

Biology

2.5K visualizações.  Teikyo University School of Medicine. As células-tronco do câncer de ovário (OCSC) são responsáveis pelo início, recorrência, resistência terapêutica e metástase do câncer. Considera-se que o nicho vascular do OCSC promove a auto-renovação dos OCSCs, levando à quimiorresistência. Este protocolo fornece a base para o estabelecimento de um modelo de nicho vascular OCSC reprodutível in vitro.

Vídeo: Evaluating the Angiogenetic Properties of Ovarian Cancer Stem-Like Cells using the Three-Dimensional Co-Culture System, NICO-1

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

The three-dimensional folding of chromosomes compartmentalizes the genome and and can bring distant functional elements, such as promoters and enhancers, into close spatial proximity 2-6. Deciphering the relationship between chromosome organization and genome activity will aid in understanding genomic processes, like transcription and replication. However, little is known about how chromosomes fold. Microscopy is unable to distinguish large numbers of loci simultaneously or at high resolution. To date, the detection of chromosomal interactions using chromosome conformation capture (3C) and its subsequent adaptations required the choice of a set of target loci, making genome-wide studies impossible 7-10.
We developed Hi-C, an extension of 3C that is capable of identifying long range interactions in an unbiased, genome-wide fashion. In Hi-C, cells are fixed with formaldehyde, causing interacting loci to be bound to one another by means of covalent DNA-protein cross-links. When the DNA is subsequently fragmented with a restriction enzyme, these loci remain linked. A biotinylated residue is incorporated as the 5' overhangs are filled in. Next, blunt-end ligation is performed under dilute conditions that favor ligation events between cross-linked DNA fragments. This results in a genome-wide library of ligation products, corresponding to pairs of fragments that were originally in close proximity to each other in the nucleus. Each ligation product is marked with biotin at the site of the junction. The library is sheared, and the junctions are pulled-down with streptavidin beads. The purified junctions can subsequently be analyzed using a high-throughput sequencer, resulting in a catalog of interacting fragments.
Direct analysis of the resulting contact matrix reveals numerous features of genomic organization, such as the presence of chromosome territories and the preferential association of small gene-rich chromosomes. Correlation analysis can be applied to the contact matrix, demonstrating that the human genome is segregated into two compartments: a less densely packed compartment containing open, accessible, and active chromatin and a more dense compartment containing closed, inaccessible, and inactive chromatin regions. Finally, ensemble analysis of the contact matrix, coupled with theoretical derivations and computational simulations, revealed that at the megabase scale Hi-C reveals features consistent with a fractal globule conformation.

The Hi-C method allows unbiased, genome-wide identification of chromatin interactions (1). Hi-C couples proximity ligation and massively parallel sequencing. The resulting data can be used to study genomic architecture at multiple scales: initial results identified features such as chromosome territories, segregation of open and closed chromatin, and chromatin structure at the megabase scale.

Oi-C: um método para estudar a arquitetura tridimensional de Genomas.

The three-dimensional folding of chromosomes compartmentalizes the genome and and can bring distant functional elements, such as promoters and enhancers, ...

Biologia

Flow Cytometry Purification of Mouse Meiotic Cells

The heterogeneous nature of cell types in the testis and the absence of meiotic cell culture models have been significant hurdles to the study of the unique differentiation programs that are manifest during meiosis. Two principal methods have been developed to purify, to varying degrees, various meiotic fractions from both adult and immature animals: elutriation or Staput (sedimentation) using BSA and/or percoll gradients. Both of these methods rely on cell size and density to separate meiotic cells1-5. Overall, except for few cell populations6, these protocols fail to yield sufficient purity of the numerous meiotic cell populations that are necessary for detailed molecular analyses. Moreover, with such methods usually one type of meiotic cells can be purified at a given time, which adds an extra level of complexity regarding the reproducibility and homogeneity when comparing meiotic cell samples.
Here, we describe a refined method that allows one to easily visualize, identify, and purify meiotic cells, from germ cells to round spermatids, using FACS combined with Hoechst 33342 staining7,8. This method provides an overall snapshot of the entire meiotic process and allows one to highly purify viable cells from most stage of meiosis. These purified cells can then be analyzed in detail for molecular changes that accompany progression through meiosis, for example changes in gene expression9,10and the dynamics of nucleosome occupancy at hotspots of meiotic recombination11.

An efficient method to obtain highly purified viable meiotic fractions from mouse testis is described, which combines a refined cell dissociation protocol with fluorescent activated cell sorting (FACS). This method takes advantage of differences in the DNA content and nuclear density of discrete meiotic fractions.

Purificação de Citometria de Fluxo de células de camundongo meiótica

The heterogeneous nature of cell types in the testis and the absence of meiotic cell culture models have been significant hurdles to the study of the ...

Isolation of Mammary Epithelial Cells from Three-dimensional Mixed-cell Spheroid Co-culture

While enormous efforts have gone into identifying signaling pathways and molecules involved in normal and malignant cell behaviors1-2, much of this work has been done using classical two-dimensional cell culture models, which allow for easy cell manipulation. It has become clear that intracellular signaling pathways are affected by extracellular forces, including dimensionality and cell surface tension3-4. Multiple approaches have been taken to develop three-dimensional models that more accurately represent biologic tissue architecture3. While these models incorporate multi-dimensionality and architectural stresses, study of the consequent effects on cells is less facile than in two-dimensional tissue culture due to the limitations of the models and the difficulty in extracting cells for subsequent analysis.
The important role of the microenvironment around tumors in tumorigenesis and tumor behavior is becoming increasingly recognized4. Tumor stroma is composed of multiple cell types and extracellular molecules. During tumor development there are bidirectional signals between tumor cells and stromal cells5. Although some factors participating in tumor-stroma co-evolution have been identified, there is still a need to develop simple techniques to systematically identify and study the full array of these signals6. Fibroblasts are the most abundant cell type in normal or tumor-associated stromal tissues, and contribute to deposition and maintenance of basement membrane and paracrine growth factors7.
Many groups have used three dimensional culture systems to study the role of fibroblasts on various cellular functions, including tumor response to therapies, recruitment of immune cells, signaling molecules, proliferation, apoptosis, angiogenesis, and invasion8-15. We have optimized a simple method for assessing the effects of mammary fibroblasts on mammary epithelial cells using a commercially available extracellular matrix model to create three-dimensional cultures of mixed cell populations (co-cultures)16-22. With continued co-culture the cells form spheroids with the fibroblasts clustering in the interior and the epithelial cells largely on the exterior of the spheroids and forming multi-cellular projections into the matrix. Manipulation of the fibroblasts that leads to altered epithelial cell invasiveness can be readily quantified by changes in numbers and length of epithelial projections23. Furthermore, we have devised a method for isolating epithelial cells out of three-dimensional co-culture that facilitates analysis of the effects of fibroblast exposure on epithelial behavior. We have found that the effects of co-culture persist for weeks after epithelial cell isolation, permitting ample time to perform multiple assays. This method is adaptable to cells of varying malignant potential and requires no specialized equipment. This technique allows for rapid evaluation of in vitro cell models under multiple conditions, and the corresponding results can be compared to in vivo animal tissue models as well as human tissue samples.

A simple method is described for analyzing effects of tissue fibroblasts on associated epithelial cells. The combination of this method and three-dimensional tissue culture can facilitate analysis of cells after isolation from 3D. The technique is applicable to cells of varying malignant potential, allowing systematic study of effects of tumor-associated stroma on tumor cells.

Isolamento de células epiteliais mamárias de tridimensional Mixed-célula esferoidal co-cultura

While enormous efforts have gone into identifying signaling pathways and molecules involved in normal and malignant cell behaviors1-2, much of this work ...

An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images

The most common software analysis tools available for measuring fluorescence images are for two-dimensional (2D) data that rely on manual settings for inclusion and exclusion of data points, and computer-aided pattern recognition to support the interpretation and findings of the analysis. It has become increasingly important to be able to measure fluorescence images constructed from three-dimensional (3D) datasets in order to be able to capture the complexity of cellular dynamics and understand the basis of cellular plasticity within biological systems. Sophisticated microscopy instruments have permitted the visualization of 3D fluorescence images through the acquisition of multispectral fluorescence images and powerful analytical software that reconstructs the images from confocal stacks that then provide a 3D representation of the collected 2D images. Advanced design-based stereology methods have progressed from the approximation and assumptions of the original model-based stereology1 even in complex tissue sections2. Despite these scientific advances in microscopy, a need remains for an automated analytic method that fully exploits the intrinsic 3D data to allow for the analysis and quantification of the complex changes in cell morphology, protein localization and receptor trafficking. 
Current techniques available to quantify fluorescence images include Meta-Morph (Molecular Devices, Sunnyvale, CA) and Image J (NIH) which provide manual analysis. Imaris (Andor Technology, Belfast, Northern Ireland) software provides the feature MeasurementPro, which allows the manual creation of measurement points that can be placed in a volume image or drawn on a series of 2D slices to create a 3D object. This method is useful for single-click point measurements to measure a line distance between two objects or to create a polygon that encloses a region of interest, but it is difficult to apply to complex cellular network structures. Filament Tracer (Andor) allows automatic detection of the 3D neuronal filament-like however, this module has been developed to measure defined structures such as neurons, which are comprised of dendrites, axons and spines (tree-like structure). This module has been ingeniously utilized to make morphological measurements to non-neuronal cells3, however, the output data provide information of an extended cellular network by using a software that depends on a defined cell shape rather than being an amorphous-shaped cellular model. To overcome the issue of analyzing amorphous-shaped cells and making the software more suitable to a biological application, Imaris developed Imaris Cell. This was a scientific project with the Eidgen&ouml;ssische Technische Hochschule, which has been developed to calculate the relationship between cells and organelles. While the software enables the detection of biological constraints, by forcing one nucleus per cell and using cell membranes to segment cells, it cannot be utilized to analyze fluorescence data that are not continuous because ideally it builds cell surface without void spaces. To our knowledge, at present no user-modifiable automated approach that provides morphometric information from 3D fluorescence images has been developed that achieves cellular spatial information of an undefined shape (Figure 1). 
We have developed an analytical platform using the Imaris core software module and Imaris XT interfaced to MATLAB (Mat Works, Inc.). These tools allow the 3D measurement of cells without a pre-defined shape and with inconsistent fluorescence network components. Furthermore, this method will allow researchers who have extended expertise in biological systems, but not familiarity to computer applications, to perform quantification of morphological changes in cell dynamics.

We developed a software platform that utilizes Imaris Neuroscience, ImarisXT and MATLAB to measure the changes in morphology of an undefined shape taken from three-dimensional confocal fluorescence of single cells. This novel approach can be used to quantify changes in cell shape following receptor activation and therefore represents a possible additional tool for drug discovery.

Uma ferramenta analítica que quantifica Alterações morfologia celular de imagens tridimensionais de fluorescência

The most common software analysis tools available for measuring fluorescence images are for two-dimensional (2D) data that rely on manual settings for ...

Using High Resolution Computed Tomography to Visualize the Three Dimensional Structure and Function of Plant Vasculature

High resolution x-ray computed tomography (HRCT) is a non-destructive diagnostic imaging technique with sub-micron resolution capability that is now being used to evaluate the structure and function of plant xylem network in three dimensions (3D) (e.g. Brodersen et al. 2010; 2011; 2012a,b). HRCT imaging is based on the same principles as medical CT systems, but a high intensity synchrotron x-ray source results in higher spatial resolution and decreased image acquisition time. Here, we demonstrate in detail how synchrotron-based HRCT (performed at the Advanced Light Source-LBNL Berkeley, CA, USA) in combination with Avizo software (VSG Inc., Burlington, MA, USA) is being used to explore plant xylem in excised tissue and living plants. This new imaging tool allows users to move beyond traditional static, 2D light or electron micrographs and study samples using virtual serial sections in any plane. An infinite number of slices in any orientation can be made on the same sample, a feature that is physically impossible using traditional microscopy methods.
Results demonstrate that HRCT can be applied to both herbaceous and woody plant species, and a range of plant organs (i.e. leaves, petioles, stems, trunks, roots). Figures presented here help demonstrate both a range of representative plant vascular anatomy and the type of detail extracted from HRCT datasets, including scans for coast redwood (Sequoia sempervirens), walnut (Juglans spp.), oak (Quercus spp.), and maple (Acer spp.) tree saplings to sunflowers (Helianthus annuus), grapevines (Vitis spp.), and ferns (Pteridium aquilinum and Woodwardia fimbriata). Excised and dried samples from woody species are easiest to scan and typically yield the best images. However, recent improvements (i.e. more rapid scans and sample stabilization) have made it possible to use this visualization technique on green tissues (e.g. petioles) and in living plants. On occasion some shrinkage of hydrated green plant tissues will cause images to blur and methods to avoid these issues are described. These recent advances with HRCT provide promising new insights into plant vascular function.

High resolution x-ray computed tomography (HRCT) is a non-destructive diagnostic imaging technique that can be used to study the structure and function of plant vasculature in 3D. We demonstrate how HRCT facilitates exploration of xylem networks across a wide range of plant tissues and species.

Usando tomografia de tórax para visualizar a estrutura tridimensional e Função da Usina Vasculatura

High resolution x-ray computed tomography (HRCT) is a non-destructive diagnostic imaging technique with sub-micron resolution capability that is now being ...

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

Visualization of dynamic signaling events in live cells has been a challenge. We expanded an established transient expression system, the biomolecular fluorescent complementation (BiFC) assay in tobacco epidermal cells, from testing protein-protein interaction to monitoring spatial distribution of signal transduction in plant cells. In this protocol, we used the BiFC assay to show that the interaction and the signaling between the Arabidopsis MAPKKK YODA and MAPK6 occur at the plasma membrane. When the scaffold protein BASL was co-expressed, the YODA-MAPK interaction redistributed and spatially co-polarized with CFP-BASL. This modified tobacco expression system allows for quick examination of signaling localization and dynamic changes (less than 4 days) and can accommodate multiple of fluorescent protein colors (at least three). We also presented detailed methods to quantify protein distribution (asymmetric spatial localization, or &#34;polarization&#34;) in tobacco cells. This advanced tobacco expression system has a potential to be used widely for quick testing of dynamic signaling events in live plant cells.

At the subcellular level, signaling events are dynamically modulated by developmental and environmental cues. Here we describe a protocol that employs the tobacco transient expression system to monitor dynamic protein-protein interaction and to disclose spatial organization of signal transduction in plant cells.

Imagiologia reorganização espacial de uma via de sinalização MAPK Utilizar o sistema de expressão transitória do Tabaco

Visualization of dynamic signaling events in live cells has been a challenge. We expanded an established transient expression system, the biomolecular ...

Development of an Insert Co-culture System of Two Cellular Types in the Absence of Cell-Cell Contact

The role of secreted soluble factors in the modification of cellular responses is a recurrent theme in the study of all tissues and systems. In an attempt to make straightforward the very complex relationships between the several cellular subtypes that compose multicellular organisms, in vitro techniques have been developed to help researchers acquire a detailed understanding of single cell populations. One of these techniques uses inserts with a permeable membrane allowing secreted soluble factors to diffuse. Thus, a population of cells grown in inserts can be co-cultured in a well or dish containing a different cell type for evaluating cellular changes following paracrine signaling in the absence of cell-cell contact. Such insert co-culture systems offer various advantages over other co-culture techniques, namely bidirectional signaling, conserved cell polarity and population-specific detection of cellular changes. In addition to being utilized in the field of inflammation, cancer, angiogenesis and differentiation, these co-culture systems are of prime importance in the study of the intricate relationships that exist between the different cellular subtypes present in the central nervous system, particularly in the context of neuroinflammation. This article offers general methodological guidelines in order to set up an experiment in order to evaluating cellular changes mediated by secreted soluble factors using an insert co-culture system. Moreover, a specific protocol to measure the neuroinflammatory effects of cytokines secreted by lipopolysaccharide-activated N9 microglia on neuronal PC12 cells will be detailed, offering a concrete understanding of insert co-culture methodology.

In multicellular organisms, secreted soluble factors elicit responses from different cell types as a result of paracrine signaling. Insert co-culture systems offer a simple way to assess the changes mediated by secreted soluble factors in the absence of cell-cell contact.

Desenvolvimento de um Sistema Insert Co-cultura de dois tipos celulares na ausência de célula-célula Contact

The role of secreted soluble factors in the modification of cellular responses is a recurrent theme in the study of all tissues and systems. In an attempt ...

Three-dimensional Reconstruction of the Vascular Architecture of the Passive CLARITY-cleared Mouse Ovary

The ovary is the main organ of the female reproductive system and is essential for the production of female gametes and for controlling the endocrine system, but the complex structural relationships and three-dimensional (3D) vasculature architectures of the ovary are not well described. In order to visualize the 3D connections and architecture of blood vessels in the intact ovary, the first important step is to make the ovary optically clear. In order to avoid tissue shrinkage, we used the hydrogel fixation-based passive CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging/ Immunostaining/In situ-hybridization-compatible Tissue Hydrogel) protocol method to clear an intact ovary. Immunostaining, advanced multiphoton confocal microscopy, and 3D image-reconstructions were then used for the visualization of ovarian vessels and follicular capillaries. Using this approach, we showed a significant positive correlation (P &#60;0.01) between the length of the follicular capillaries and volume of the follicular wall.

Here we present an adaptation of the passive CLARITY and 3D reconstruction method for visualization of the ovarian vasculature and follicular capillaries in intact mouse ovaries.

Reconstrução tridimensional da arquitetura Vascular do ovário passiva Mouse clareza-limpo

The ovary is the main organ of the female reproductive system and is essential for the production of female gametes and for controlling the endocrine ...

Adipo-Clear: A Tissue Clearing Method for Three-Dimensional Imaging of Adipose Tissue

Adipose tissue plays a central role in energy homeostasis and thermoregulation. It is composed of different types of adipocytes, as well as adipocyte precursors, immune cells, fibroblasts, blood vessels, and nerve projections. Although the molecular control of cell type specification and how these cells interact have been increasingly delineated, a more comprehensive understanding of these adipose-resident cells can be achieved by visualizing their distribution and architecture throughout the whole tissue. Existing immunohistochemistry and immunofluorescence approaches to analyze adipose histology rely on thin paraffin-embedded sections. However, thin sections capture only a small portion of tissue; as a result, the conclusions can be biased by what portion of tissue is analyzed. We have therefore developed an adipose tissue clearing technique, Adipo-Clear, to permit comprehensive three-dimensional visualization of molecular and cellular patterns in whole adipose tissues. Adipo-Clear was adapted from iDISCO/iDISCO+, with specific modifications made to completely remove the lipid stored in the tissue while preserving native tissue morphology. In combination with light-sheet fluorescence microscopy, we demonstrate here the use of the Adipo-Clear method to obtain high-resolution volumetric images&#160;of an entire adipose tissue.

Due to the high lipid content, adipose tissue has been challenging to visualize using traditional histological methods. Adipo-Clear is a tissue clearing technique that allows robust labeling and high-resolution volumetric fluorescent imaging of adipose tissue. Here, we describe the methods for sample preparation, pretreatment, staining, clearing, and mounting for imaging.

Adipo-Clear: Um método de compensação por imagem tridimensional do tecido adiposo tecido

Adipose tissue plays a central role in energy homeostasis and thermoregulation. It is composed of different types of adipocytes, as well as adipocyte ...

Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

Organoid technology, in vitro 3D culturing of miniature tissue, has opened a new experimental window for cellular processes that govern organ development and function as well as disease. Fluorescence microscopy has played a major role in characterizing their cellular composition in detail and demonstrating their similarity to the tissue they originate from. In this article, we present a comprehensive protocol for high-resolution 3D imaging of whole organoids upon immunofluorescent labeling. This method is widely applicable for imaging of organoids differing in origin, size and shape. Thus far we have applied the method to airway, colon, kidney, and liver organoids derived from healthy human tissue, as well as human breast tumor organoids and mouse mammary gland organoids. We use an optical clearing agent, FUnGI, which enables the acquisition of whole 3D organoids with the opportunity for single-cell quantification of markers. This three-day protocol from organoid harvesting to image analysis is optimized for 3D imaging using confocal microscopy.

The entire 3D structure and cellular content of organoids, as well as their phenotypic resemblance to the original tissue can be captured using the single-cell resolution 3D imaging protocol described here. This protocol can be applied to a wide range of organoids varying in origin, size and shape.

Resolução unicelular imagens tridimensionais de organoides intactos

Organoid technology, in vitro 3D culturing of miniature tissue, has opened a new experimental window for cellular processes that govern organ development ...