Human Immortalized Skin Keratinocytes (hKCs) Monolayer Seeding
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Results: Characterization of Skin Model in The Microfluidic Chip with Confocal and Fluorescence Microscopy
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Conclusion
TRANSCRIÇÃO
This is a novel, lithography-free protocol for modeling a 3D skin-on-chip using micromachined, adhesive vinyl layers and a parallel-flow methodology for the dermal compartment generation. Micromachined vinyl provides more simplicity in the fabrica
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Here, we present a protocol to generate a three-dimensional simplified and undifferentiated skin model using a micromachined microfluidic platform. A parallel flow approach allows the in situ deposition of a dermal compartment for the seeding of epithelial cells on top, all controlled by syringe pumps.