Microtransplantation of Synaptic Membranes to Reactivate Human Synaptic Receptors for Functional Studies

2.1K Views

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10:08 min

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July 20th, 2022

DOI :

10.3791/64024-v

July 20th, 2022

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2,053 Views

Brice Miller*¹, Ashli Powell*¹, Berenice A. Gutierrez¹, Agenor Limon¹

¹The Mitchell Center for Neurodegenerative Diseases, Department of Neurology, University of Texas Medical Branch

Capítulos

0:04

Introduction

1:13

Loading the Sample

2:06

Injecting the Oocytes

2:49

Recording of Ion Currents Using a Two-Electrode Voltage Clamp

6:50

Analyzing TEVC Recordings

7:30

Results: Representative Ion Currents of Oocytes Microtransplanted with Human Synaptic Receptors and Polarized Insertion of Receptors

9:25

Conclusion

Transcrição

Neurodegenerative and mental health disorders result from changes in synaptic communication. Multifactorial events change synaptic receptors in ways that we do not completely understand. Microtransplantation of synaptic membranes provides insight

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Resumo

The protocol demonstrates that by performing microtransplantation of synaptic membranes into Xenopus laevis oocytes, it is possible to record consistent and reliable responses of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid and γ-aminobutyric acid receptors.

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