Recording of Ion Currents Using a Two-Electrode Voltage Clamp
6:50
Analyzing TEVC Recordings
7:30
Results: Representative Ion Currents of Oocytes Microtransplanted with Human Synaptic Receptors and Polarized Insertion of Receptors
9:25
Conclusion
필기록
Neurodegenerative and mental health disorders result from changes in synaptic communication. Multifactorial events change synaptic receptors in ways that we do not completely understand. Microtransplantation of synaptic membranes provides insight
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The protocol demonstrates that by performing microtransplantation of synaptic membranes into Xenopus laevis oocytes, it is possible to record consistent and reliable responses of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid and γ-aminobutyric acid receptors.