High-Throughput Screening of Hsp90 Inhibitors by the Malachite Green Assay: Assay Design and Addition Orde
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Results: Reactions of Malachite Green Reagent, Standard Plot for Standard Phosphate Concentration, and IC50 Calculation for Geldanamycin Against Yeast Hsp90
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Conclusion
Transcrição
This protocol will aid in the determination of the activity for ATPase proteins by measuring the free phosphate resulting from ATP hydrolysis by the proteins. The malachite green assay is a simple, sensitive, fast, and inexpensive procedure for th
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The malachite green assay protocol is a simple and cost-effective method to discover heat shock protein 90 (Hsp90) suppressors, as well as other inhibitor compounds against ATP-dependent enzymes.