Methods to Enable Spatial Transcriptomics of Bone Tissues
2.5K Views
•
07:43 min
•
May 3rd, 2024
DOI :
May 3rd, 2024
•Luigi Mancinelli1,2, Karen Elizabeth Schoedel3,4, Kurt Richard Weiss5,6,7, Giuseppe Intini1,2,8,9,10
1Department of Periodontics and Preventive Dentistry, University of Pittsburgh School of Dental Medicine, 2Center for Craniofacial Regeneration, University of Pittsburgh School of Dental Medicine, 3UPMC Presbyterian, 4UPMC Shadyside, 5Musculoskeletal Oncology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh, 6UPMC Hillman Cancer Center, 7Departments of Anatomic Pathology and General Surgical Oncology, University of Pittsburgh, 8Department of Medicine, University of Pittsburgh School of Medicine, 9University of Pittsburgh UPMC Hillman Cancer Center, 10McGowan Institute for Regenerative Medicine, University of Pittsburgh
Chapters in this video
0:00
Introduction
1:11
Isolation and Sectioning of Bone Tissue from Mouse for Transcriptomic Studies
Related Videos
08:58
An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples
9.2K Views
10:43
Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration
6.7K Views
11:06
Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches
7.9K Views
09:19
Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
4.8K Views
09:20
Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
7.0K Views
07:17
Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation
83.2K Views
Spatial High-resolution Analysis of Gene Expression Levels in Tendons
299 Views
04:20
Practical Considerations for the Design, Execution, and Interpretation of Studies Involving Whole-Bone Bending Tests of Rodent Bones
741 Views
06:59
Trabecular Bone Microarchitecture Evaluation in an Osteoporosis Mouse Model
2.2K Views
10:11
A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones
9.5K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved