Генерация геномных делеций в линии клеток млекопитающих, через CRISPR / Cas9

1:22

Transfecting CRISPRs into MEL Cells

2:35

Fluorescence Activated Cell Sorting of Transfected Cells

3:41

Primer Validation and Screening Bulk Cultures for CRISPR/Cas9-Mediated Deletion

5:26

Screening CRISPR/Cas9 Clones for Deletions and Clone Selection

7:10

Results: Deletion of Pim1 in MEL Cells

9:00

Conclusion