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Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

95.2K Views

09:40 min

January 3rd, 2015

DOI :

10.3791/52118-v

January 3rd, 2015


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Genomic Deletions

Chapters in this video

0:05

Title

1:22

Transfecting CRISPRs into MEL Cells

2:35

Fluorescence Activated Cell Sorting of Transfected Cells

3:41

Primer Validation and Screening Bulk Cultures for CRISPR/Cas9-Mediated Deletion

5:26

Screening CRISPR/Cas9 Clones for Deletions and Clone Selection

7:10

Results: Deletion of Pim1 in MEL Cells

9:00

Conclusion

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