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Abstract

Biology

A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies

Published: August 13th, 2020

DOI:

10.3791/61617

1Division of Vaccine Discovery, La Jolla Institute for Immunology

ERRATUM NOTICE

Important: There has been an erratum issued for this article. Read more …

Abstract

Chromatin immunoprecipitation followed by sequencing (ChIP-Seq) is a powerful and widely used approach to profile chromatin DNA associated with specific histone modifications, such as H3K27ac, to help identify cis-regulatory DNA elements. The manual process to complete a ChIP-Seq is labor intensive, technically challenging, and often requires large-cell numbers (>100,000 cells). The method described here helps to overcome those challenges. A complete semiautomated, microscaled H3K27ac ChIP-Seq procedure including cell fixation, chromatin shearing, immunoprecipitation, and sequencing library preparation, for batch of 48 samples for cell number inputs less than 100,000 cells is described in detail. The semiautonomous platform reduces technical variability, improves signal-to-noise ratios, and drastically reduces labor. The system can thereby reduce costs by allowing for reduced reaction volumes, limiting the number of expensive reagents such as enzymes, magnetic beads, antibodies, and hands-on time required. These improvements to the ChIP-Seq method suit perfectly for large-scale epigenetic studies of clinical samples with limited cell numbers in a highly reproducible manner.

Erratum

Erratum: A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies

An erratum was issued for: A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies. An author's name was updated.

The name was corrected from:

Pandurangan Vijayanad

to:

Pandurangan Vijayanand

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Keywords ChIP Seq

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