Begin by opening the MVS software application on the TEM instrument and selecting other. Connect the microscope to the MVS software. Navigate to the image metadata tab and select magnification, max dose and dose rate to overlay on the image stream seen in the live display.
Other metadata may be included if the user desires. Open the dose tab. Select dose management and enable dose monitoring.
Then select show dose layer and show position uncertainty to activate automated electron dose tracking and display the dose color overlay. Set the values for the low dose level and high dose level before pressing save. Then navigate to the settings tab.
Select dose and set the dose navigation map opacity and dose image overlay opacity values. Activate drift correction in the live image viewer window by clicking on drift correct. Next, navigate to the data view tab and plot the metadata values defocus and focus quotient on the y axis.
Activate focus assist then select calibrate focus to run the calibration of the automated focus assist. Once the calibrate focus routine is complete close the data view tab. In the MVS software, open the image analysis tab and activate the live FFT and quadrants one and two options.
Using the microscope software controls adjust the beam conditions so that the electron flux is approximately 600 angstrom square per second. Move to a new region in the sample and center the sample ROI in the live view of the MVS software. Maintain a constant dose rate while continuously imaging the same ROI until the spots corresponding to the atomic structure and the FFT have disappeared.
