The authors declare that they have no competing financial interests.

Hayvanlar, bariyer patojenden ari bir yere yerleştirilmiştir. Bütün hayvanlar Ulusal tarafından Tıbbi Araştırmalar Ulusal Derneği &quot;ve&quot; Ulusal Bilimler Akademisi tarafından hazırlanan Laboratuvar Hayvanları &quot;Bakımı ve Kullanımı Kılavuzuna tarafından formüle yayınlanan&quot; Laboratuar Hayvan Bakımı Esasları &quot;uyarınca insan bakım almak Sağlık Enstitüleri (NIH Yayın yok. 86-23, 1985 revize). Tüm deneyler Eğitim, Bilim ve Kültür Avusturya Maliye Bakanlığı tarafından onaylanır. 1. Alıcı Hazırlık <ol><li> Xylazine (5 mg / kg vücut ağırlığı) ve ketamin (100 mg / kg vücut ağırlığı) bir İM enjeksiyonu ile alıcı hayvan anestezisi. </li><li> Hayvanın yan servikal bölgedeki tüm saç çıkarın ve operatif field chlorhexdine kullanarak üç kez fırçalayın. </li><li> Operatif sahada yatar pozisyonda fare yerleştirin. </li><li> Sonraki boyun inc bir cilt kesi yapmaksağ alt çeneye izyonu. </li><li> Daha sonra, açık açık, sağ eksternal juguler ven (EJV) harekete geçirmek ve ligatürlerin arasındaki dalları bölmek. </li><li> Sonra manşet vücut üzerinde proksimal güdük tersine çevrilmesi için yeterli uzunlukta bırakın emin ligatures arasındaki EJV bölmek. </li><li> Sonraki submandibular bezin sağ lobunu çıkarın. </li><li> Venöz anastomoz için polietilen manşet aracılığıyla EJV proksimal ucunu geçmek ve bir mikro kelepçe ile manşet sapına bunu düzeltmek. </li><li> , Geminin sonunda ligatürün çıkarın manşet lümen tersine çevrilmesi ve 8-0 ipek döngü (Şekil 1a) ile sabitleyin. </li><li> Ardından karotis arter erişmek için bipolar koter ile sağ SKM kas transect. </li><li> Sonraki karotis arter harekete geçirmek ve bitişik harfler arasındaki gemi kesti. </li><li> Sonraki karotis arter harekete geçirmek ve bitişik harfler arasındaki gemi kesti. </li><li> Dis geçmektal manşet yoluyla geminin sonu ve arter kelepçe ile sabitleyin. </li><li> Teknenin sonunda bitişik harfleri çıkarın ve vasküler dilatatörler kullanılarak gerilmek lümeni. Sonraki, EJV benzer şekilde, manşet arter ters çevrilecek ve 8-0 ipek döngü ile sabitleyin. </li></ol> 2. Kalp İhale <ol><li> Xylazine (5 mg / kg vücut ağırlığı) ve ketamin (100 mg / kg vücut ağırlığı) bir İM enjeksiyonu ile verici fare anestezisi. Daha sonra hayvanın karın bölgesi tüm tüylerden. Chlorhexdine kullanarak ameliyat alan üç kez fırçalamaktadır. Operatif alanın üzerine sırt üstü yatırıldı fare daha önce anlatıldığı gibi sonraki bir yer. </li><li> Bir orta hat karın kesi ardından, inferior vena kava (IVC) ortaya çıkarmak için sola Q-ipuçları iç organları geri çekin. </li><li> Heparinizasyon için IVC içine 4 heparin- natriyumhidroksid çözümü: 1 400 ul enjekte edilir. </li><li> Acc kazanmak için cranially üzerinde ön göğüs duvarına bir torakotomi gerçekleştirin ve katkalbe ess. </li><li> Sonraki, timus çıkarın ve sol ve sağ superior vena kava kesiler havalandırma yapmak. </li><li> Daha sonra, brakiosefalik şube düzeyinde bir 27 G şırınga ile aort arkı kanül ile 4 ° Celsius HTK solüsyonu ile retrograd kalp serpmek. </li><li> 8-0 ipek ile alt ve üst vena kava bağlanır ve bitişik harfler distal onları bölmek. </li><li> Sonraki önceki kanül seviyesinde aort arkı bölmek ve pulmoner gövde serbest incelemek ve mümkün olduğunca distale bölmek. </li><li> Bunun ardından, bir yığın ligasyonu ile pulmoner venlerin kravat ve bitişik harfler distal onları bölmek. </li><li> Son olarak, donör sitesinden kalp çıkarın ve 4 ° Celsius HTK solüsyonu (Şekil 1b) saklayın. </li></ol> 3. İmplantasyon <ol><li> Bir baş aşağı konumda alıcı boyun bölgesinde kalp greft yerleştirin (Şekil 1c </strong&gt;). </li><li> Sonraki alıcının ven manşet yapı üzerinde kalp akciğer gövde çekin ve bir ipek döngü (Şekil 1d) ile sabitleyin. </li><li> Greft aorta ve aynı moda (Şekil 1e) olarak alıcı hayvanın everte arter arasındaki anastomoz gerçekleştirin. </li><li> Daha sonra, arteryel kelepçe ardından ilk venöz kelepçe, kaldırın. Kalp reperfuzyona ve 1-2 dakika içinde yenerek başlar. Reperfüzyon sırasında sıcak (35 ° C) tuzlu su ile kalp nemlendirin. </li><li> Son olarak, sürekli 6-0 dikişler (Şekil 1e) ile cerrahi yara kapatın. </li></ol> 4. Ameliyat Sonrası Bakım, Endpoint <ol><li> Sıvı replasmanı için postoperatif 0.3 ml normal tuzlu ip vazgeçtim. </li><li> Anesteziden awaking kadar bir ısı lambası altında hayvan yerleştirin. </li><li> Uyanık, onlar yiyecek ve suya serbestçe ulaşabilir barınak ortamına dönüş hayvan. </li><li> MIilk 7 gün boyunca ameliyat sonrası ağrı durumuna küçült Karprofen (4 mg / kg her 12 saatte subkutan (sc), tatbik. Ek olarak, 5 gün süre ile hemen işleminden sonra) Buprenorphin her 12 saat (0.1 mg / kg sağlar. </li><li> Doğru besin alımını değerlendirmek için her alıcı hayvan haftalık ağırlığını (g) edinin. Daha fazla% 10-15 kilo kaybı bulgusu cerrahi, güncel, ilgisizlik, sakatlayıcı veya çok bükülmüş arka ağırlığına kıyasla varsa, klinik bitiş noktasına ulaşmadan önce, terminal izofluran inhalasyon kullanmadan hayvan kurban. Kalp reddedildi sonra da hayvan kurban. </li></ol>

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H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+technique+of+cervical+heterotopic+heart+transplantation+in+mice.">A technique of cervical heterotopic heart transplantation in mice.</a> Transplantation. 52, 1099-1101 (1991).</li><li>Huang, X., Chen, D., Chen, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=[A+modified+model+of+cervical+heterotopic+cardiac+transplantation+for+chronic+rejection+research].">[A modified model of cervical heterotopic cardiac transplantation for chronic rejection research].</a> Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 22, 1508-1510 (2008).</li><li>Hasegawa, T., Visovatti, S. H., Hyman, M. C., Hayasaki, T., Pinsky, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Heterotopic+vascularized+murine+cardiac+transplantation+to+study+graft+arteriopathy.">Heterotopic vascularized murine cardiac transplantation to study graft arteriopathy.</a> Nat Protoc. 2, 471-480 (2007).</li><li>Wang, C. Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Suppression+of+murine+cardiac+allograft+arteriopathy+by+long-term+blockade+of+CD40-CD154+interactions.">Suppression of murine cardiac allograft arteriopathy by long-term blockade of CD40-CD154 interactions.</a> Circulation. 105, 1609-1614 (2002).</li><li>Matsuura, A., Abe, T., Yasuura, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Simplified+mouse+cervical+heart+transplantation+using+a+cuff+technique.">Simplified mouse cervical heart transplantation using a cuff technique.</a> Transplantation. 51, 896-898 (1991).</li><li>Tomita, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Improved+technique+of+heterotopic+cervical+heart+transplantation+in+mice.">Improved technique of heterotopic cervical heart transplantation in mice.</a> Transplantation. 64, 1598-1601 (1997).</li><li>Zhou, Y., Gu, X., Xiang, J., Qian, S., Chen, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+comparative+study+on+suture+versus+cuff+anastomosis+in+mouse+cervical+cardiac+transplant.">A comparative study on suture versus cuff anastomosis in mouse cervical cardiac transplant.</a> Exp Clin Transplant. 8, 245-249 (2010).</li><li>Corry, R. J., Winn, H. J., Russell, P. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Primarily+vascularized+allografts+of+hearts+in+mice.+The+role+of+H-2D,+H-2K,+and+non-H-2+antigens+in+rejection.">Primarily vascularized allografts of hearts in mice. The role of H-2D, H-2K, and non-H-2 antigens in rejection.</a> Transplantation. 16, 343-350 (1973).</li><li>Kamada, N., Calne, R. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Orthotopic+liver+transplantation+in+the+rat.+Technique+using+cuff+for+portal+vein+anastomosis+and+biliary+drainage.">Orthotopic liver transplantation in the rat. Technique using cuff for portal vein anastomosis and biliary drainage.</a> Transplantation. 28, 47-50 (1979).</li><li>Zimmermann, F. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Techniques+for+orthotopic+liver+transplantation+in+the+rat+and+some+studies+of+the+immunologic+responses+to+fully+allogeneic+liver+grafts.">Techniques for orthotopic liver transplantation in the rat and some studies of the immunologic responses to fully allogeneic liver grafts.</a> Transplant Proc. 11, 571-577 (1979).</li><li>Maglione, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Donor+pretreatment+with+tetrahydrobiopterin+saves+pancreatic+isografts+from+ischemia+reperfusion+injury+in+a+mouse+model.">Donor pretreatment with tetrahydrobiopterin saves pancreatic isografts from ischemia reperfusion injury in a mouse model.</a> Am J Transplant. 10, 2231-2240 (2010).</li><li>Heron, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+technique+for+accessory+cervical+heart+transplantation+in+rabbits+and+rats.">A technique for accessory cervical heart transplantation in rabbits and rats.</a> Actapathologica et microbiologica Scandinavica Section A, Pathology. 79, 366-372 (1971).</li></ol>

This study has been supported by grant #207 of the &#8220;Medizinischer Forschungsfond Tirol&#8221;, grant #2010022010 of the &#8220;MUI-START F&#246;rderungsprogramm&#8221; of the Innsbruck Medical University.

Vaskülarize allogreft reddi sadece yetersiz m vitro modeller kullanılarak değerlendirilebilir farklı adımları bir bolluk gerektirir. Alıcıların sensitizasyon, antijen işleme ikincil lenfoid organlarda değil, aynı zamanda, bağışıklık yetkin hücrelerin farklılaşması ve proliferasyonu gibi in vivo fenomen daha iyi karakterize edilmiş bulunmaktadır. Hayvan modelleri böylece translasyonel araştırma 16 için ideal bir araçtır. Fare modelleri hala transgenik ve gen nakav...

Kalp transplantasyonu farklı son dönem kalp hastalıklardan muzdarip hastalar için tercih tedaviyi temsil eder. Cerrahi tekniklerin, enfeksiyonların daha etkili profilaksi, ve yeni immunsupresif rejimler ilerlemeler organ nakli 6 belirgin geliştirilmiş sonucunu sonuçlandı. Ancak, uzun dönem greft sağkalım son yıllarda üzerinde 7 titizlikle geliştirilmiş değil. Nakli damar sertliği ile karakterize kronik rejeksiyon, uzun dönem greft sağkalım 8-11 önemli bir engel olmaya devam etmektedir. Farelerde heterotopik kalp nakli modeli akut dönemde immünolojik mekanizma analizi için önemli ve geçerli bir araç yanı sıra kronik rejeksiyon 12-15 sağlar. Bugüne kadar en sık nakli modeli dikiş tekniği kullanılarak karın fare kalp nakli hala. Donör kalbin çıkan aort a anastomoz edilirbdominal aort ve pulmoner arter alıcının vena kava anastomoz edilir. Sütür modelin mikrocerrahi zorluk çoğu 16,17 dikilir gemilerin, küçük boyutuna bağlıdır. Dikiş modelinin aksine kalp eksternal juguler ven pulmoner arter ve karotis arter donörün aort anastomoz edilir alıcının boyun bölgesinde yerleştirilir. Manşet tekniği kullanılarak servikal kalp nakli modelinin gelişim mantığı bu modelin geniş uygulamasını sağlayacak temel mikrocerrahi becerileri ile yüksek başarı oranları elde sağlayan bir hayvan modeli, sahip oldu. Bu yöntemin önemli bir avantajı dikiş modeli 18 ile karşılaştırıldığında kanama ve tromboz gibi komplikasyonları ile ilgili önemli ölçüde daha az meydana anastomoz bulunmaktadır.

<table border="0" cellpadding="0" cellspacing="0">
	<tbody>
		<tr>
			<td>Yasargil Clip Mini Permanent 7mm</td>
			<td>Aesculap</td>
			<td>FE720K</td>
			<td></td>
		</tr>
		<tr>
			<td>Micro vessel clip</td>
			<td>S &#38; T</td>
			<td>B1 00396 V</td>
			<td></td>
		</tr>
		<tr>
			<td>Microscissor</td>
			<td>FST</td>
			<td>14075-11</td>
			<td></td>
		</tr>
		<tr>
			<td rowspan="2">Vesseldilatator</td>
			<td rowspan="2">S &#38; T</td>
			<td rowspan="2">D-5a.2 00125</td>
			<td></td>
		</tr>
		<tr>
			<td></td>
		</tr>
		<tr>
			<td>Microforceps</td>
			<td>FST</td>
			<td>Dumont 11271- 30</td>
			<td></td>
		</tr>
		<tr>
			<td>Clipapplicator</td>
			<td>S &#38; T</td>
			<td>CAF-4 00072</td>
			<td></td>
		</tr>
		<tr>
			<td>Microvessel clip</td>
			<td>S &#38; T</td>
			<td>B1 00396 V</td>
			<td></td>
		</tr>
	</tbody>
</table>

murine cervical heart transplantation model using a modified cuff technique

<html> Fare model monoklonal antikorların çeşitli beri araştırılmaktadır özel ilgi ve ticari olarak doğuştan tanımlandığı gibidir ve boşaltma suşları in vivo çalışmalar mekanik gerçekleştirmek için kullanılabilir. Bir dikiş tekniği kullanılarak kalp nakli modeller ilk kez başarıyla sıçanlarda geliştirilen iken, aynı derecede yaygın kullanılan kemirgen eşdeğer içine çeviri mikrocerrahi işlemin teknik karmaşıklığı nedeniyle elde değildi. Buna karşılık olarak, aynı zamanda, farelerde geliştirilmişken olmayan dikiş manşet teknikleri, farelerde başarılı bir şekilde 1-3 kullanılmak üzere adapte edilmiştir. Revaskülarizasyon için bu teknik bir polietilen manşet alıcı gemiyi tersine çevrilmesi iki önemli adım I) içerir; II) eski everte alıcı geminin üzerinde donör gemiyi çekerek ve çevresel bir kravat ile yerde tutuyor. Bu endotel tabakası, kısa çalışma süresi ve çok yüksek açıklık oranlarının 4 bir sürekliliğini sağlar. t &quot;&gt; vasküler anastomoz için bu tekniği kullanarak biz karotis arter girişi arteriyel için.% 95 genel başarı oranı ile 1.000 &#39;den fazla servikal kalp nakli gerçekleştirilen ve proksimal aort arkı nakledilen kalbin bir retrograd perfüzyon sonuçlanan anastomoz edildi. venöz drenaj için greft pulmoner arter alıcının 5 eksternal juguler ven ile anastomoz edildi. Burada, videoyu tamamlamak için bu tekniğin ek ayrıntıları.

Şekil 2 tam MHC uyumsuz C57BL / 6 alıcılarının içine naklinden sonra BALB / c vericilerden kalp allogreftlerinin temsili sağkalım verileri gösterir. Kalpler nakli sonrası günde 7 ± 1 reddedilir. Nakledilen allogreftin parmak palpasyonu allogreft reddinin zamana bağlı ders tespit etmek için hassas bir yöntemdir. Dizimizin yapılan tüm singenik kalp nakilleri süresiz atlattı (&gt; 150 gün). <img alt="Şekil 1" src="/file...

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Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique

Sıçangil modeli servikal kalp nakli immünolojik hem de iskemi reperfüzyon yaralanması çalışmasından için uygundur. Biz olmayan bir dikiş manşet tekniği kullanılarak prosedür değiştirilmiş ve bu yaklaşım ile 1.000 &#39;den fazla başarılı nakli gerçekleştirildi. Burada, videoyu tamamlamak için bu tekniğin ek ayrıntıları.

Modifiye Manşet Tekniği Sıçangiller Servikal Kalp Nakli Modeli

Mouse models are of special interest in research since a wide variety of monoclonal antibodies and commercially defined inbred and knockout strains are ...

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13.7K Görüntüleme.  Innsbruck Medical University.  Sıçangil modeli servikal kalp nakli immünolojik hem de iskemi reperfüzyon yaralanması çalışmasından için uygundur. Biz olmayan bir dikiş manşet tekniği kullanılarak prosedür değiştirilmiş ve bu yaklaşım ile 1.000 'den fazla başarılı nakli gerçekleştirildi. Burada, videoyu tamamlamak için bu tekniğin ek ayrıntıları. 

Video: Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique

Murine Model of Wound Healing

Wound healing and repair are the most complex biological processes that occur in human life. After injury, multiple biological pathways become activated. Impaired wound healing, which occurs in diabetic patients for example, can lead to severe unfavorable outcomes such as amputation. There is, therefore, an increasing impetus to develop novel agents that promote wound repair. The testing of these has been limited to large animal models such as swine, which are often impractical. Mice represent the ideal preclinical model, as they are economical and amenable to genetic manipulation, which allows for mechanistic investigation. However, wound healing in a mouse is fundamentally different to that of humans as it primarily occurs via contraction. Our murine model overcomes this by incorporating a splint around the wound. By splinting the wound, the repair process is then dependent on epithelialization, cellular proliferation and angiogenesis, which closely mirror the biological processes of human wound healing. Whilst requiring consistency and care, this murine model does not involve complicated surgical techniques and allows for the robust testing of promising agents that may, for example, promote angiogenesis or inhibit inflammation. Furthermore, each mouse acts as its own control as two wounds are prepared, enabling the application of both the test compound and the vehicle control on the same animal. In conclusion, we demonstrate a practical, easy-to-learn, and robust model of wound healing, which is comparable to that of humans.

A murine model of cutaneous wound healing that can be used to assess therapeutic compounds in physiological and pathophysiological settings.

Yara Şifa murin Modeli

Wound healing and repair are the most complex biological processes that occur in human life. After injury, multiple biological pathways become activated. ...

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Murine Heterotopic Heart Transplant Technique

It is now over forty years since this technique was first reported by Corry, Wynn and Russell. Although it took some years for other labs to become proficient in and utilize this technique, it is now widely used by many laboratories around the world. A significant refinement to the original technique was developed and reported in 2001 by Niimi. Described here are the techniques that have evolved over more than a decade in the hands of three surgeons (Plenter, Grazia, Pietra) in our center. These techniques are now being passed on to a younger generation of surgeons and researchers.
Based largely on the Niimi experience, the procedures used have evolved in the fine details - details which we will endeavor to relate here in such a way that others may be able to use this very useful model. Like Niimi, we have found that a video aid to learning is a priceless resource for the beginner.

The manuscript describes the steps required to perform the heterotopic heart transplant in the mouse.

Mürin Heterotopik Kalp Nakli Tekniği

It is now over forty years since this technique was first reported by Corry, Wynn and Russell. Although it took some years for other labs to become ...

Transplantation of Pulmonary Valve Using a Mouse Model of Heterotopic Heart Transplantation

Tissue engineered heart valves, especially decellularized valves, are starting to gain momentum in clinical use of reconstructive surgery with mixed results. However, the cellular and molecular mechanisms of the neotissue development, valve thickening, and stenosis development are not researched extensively. To answer the above questions, we developed a murine heterotopic heart valve transplantation model. A heart valve was harvested from a valve donor mouse and transplanted to a heart donor mouse. The heart with a new valve was transplanted heterotopically to a recipient mouse. The transplanted heart showed its own heartbeat, independent of the recipient&#8217;s heartbeat. The blood flow was quantified using a high frequency ultrasound system with a pulsed wave Doppler. The flow through the implanted pulmonary valve showed forward flow with minimal regurgitation and the peak flow was close to 100 mm/sec. This murine model of heart valve transplantation is highly versatile, so it can be modified and adapted to provide different hemodynamic environments and/or can be used with various transgenic mice to study neotissue development in a tissue engineered heart valve.

In order to understand the cellular and molecular mechanisms underlying neotissue formation and stenosis development in tissue engineered heart valves, a murine model of heterotopic heart valve transplantation was developed. A pulmonary heart valve was transplanted to recipient using the heterotopic heart transplantation technique.

Heterotopik Kalp Nakli bir Fare Modeli Kullanarak Pulmoner Vana Nakli

Tissue engineered heart valves, especially decellularized valves, are starting to gain momentum in clinical use of reconstructive surgery with mixed ...

Murine Corneal Transplantation: A Model to Study the Most Common Form of Solid Organ Transplantation

Corneal transplantation is the most common form of organ transplantation in the United States with between 45,000 and 55,000 procedures performed each year. While several animal models exist for this procedure and mice are the species that is most commonly used. The reasons for using mice are the relative cost of using this species, the existence of many genetically defined strains that allow for the study of immune responses, and the existence of an extensive array of reagents that can be used to further define responses in this species. This model has been used to define factors in the cornea that are responsible for the relative immune privilege status of this tissue that enables corneal allografts to survive acute rejection in the absence of immunosuppressive therapy. It has also been used to define those factors that are most important in rejection of such allografts. Consequently, much of what we know concerning mechanisms of both corneal allograft acceptance and rejection are due to studies using a murine model of corneal transplantation. In addition to describing a model for acute corneal allograft rejection, we also present for the first time a model of late-term corneal allograft rejection.

Mice have been used as a model for studying many forms of transplantation, including corneal transplantation. We describe in this report a murine model for both acute and late-term corneal transplantation.

Sıçangiller Kornea Nakli: Katı Organ Nakli En Yaygın Formu Eğitim için Bir Model

Corneal transplantation is the most common form of organ transplantation in the United States with between 45,000 and 55,000 procedures performed each ...

Heterotopic Cervical Heart Transplantation in Mice

The heterotopic cervical heart transplantation in mice is a valuable tool in transplant and cardiovascular research. The cuff technique greatly simplifies this model by avoiding challenging suture anastomoses of small vessels thereby reducing warm ischemia time. In comparison to abdominal graft implantation the cervical model is less invasive and the implanted graft is easily accessible for further follow-up examinations. Anastomoses are performed by pulling the ascending aorta of the graft over the cuff with the recipient&#8217;s common carotid artery and by pulling the main pulmonary artery over the cuff with the external jugular vein. Selection of appropriate cuff size and complete mobilization of the vessels are important for successful revascularization. Ischemia-reperfusion (I/R) injury can be minimized by perfusing the graft with a cardioplegic solution and by hypothermia. In this article, we provide technical details for a simplified and improved cuff technique, which should allow surgeons with basic microsurgical skills to perform the procedure with a high success rate.

The cuff technique shortens and facilitates the model of heterotopic cervical heart transplantation in mice by avoiding technically challenging suture anastomoses of small vessels. The technical details shown in this video paper should allow researches to establish this model in their laboratories.

Farelerde Heterotopik Servikal Kalp Nakli

The heterotopic cervical heart transplantation in mice is a valuable tool in transplant and cardiovascular research. The cuff technique greatly simplifies ...

Using the Sleeve Technique in a Mouse Model of Aortic Transplantation - An Instructional Video

Orthotopic aortic transplantation using the sleeve technique reduces injury to the aorta with failure rate of only 10-20%. The time to anastomose the aorta in mice using the sleeve method was short and easy averaging 20 min, permitting studies of iso/allo grafts. The following article describes the aortic transplantation procedure used in our laboratory. The mice were anesthetized with a mixture of 1.5% volume isoflurane and 100% oxygen through a face mask. At this point, the segment of the aorta between the renal arteries and its bifurcation was separated from the vena cava, freely prepared and clampedat the proximal and distal segments with a single silk suture. Prior to the removal of the aorta, a saline solution containing heparin was injected into the inferior vena cava. Then the aorta was cut between the clamps and a saline heparin solution was used to flush the lumen. The sleeve technique with monofilament sutures was used in order to transplant the abdominal aorta in the orthotopic position.

We present an orthotopic aortic transplantation model using the sleeve technique in mice. It is a very rapid anastomosis method, which can be employed in studies of vascular disease.

Kol tekniği aort transplantasyonu - eğitici bir Video bir fare Model kullanma

Orthotopic aortic transplantation using the sleeve technique reduces injury to the aorta with failure rate of only 10-20%. The time to anastomose the ...

Mouse Model for Pancreas Transplantation Using a Modified Cuff Technique

Mouse models have several advantages in transplantation research, including easy handling, a variety of genetically well-defined strains, and the availability of the widest range of molecular probes and reagents to perform in vivo as well as in vitro studies. Based on our experience with various murine transplantation models, we developed a heterotopic pancreas transplantation model in mice with the intent to analyze mechanisms underlying severe ischemia reperfusion injury-associated early graft damage. In contrast to previously described techniques using suture techniques, herein we describe a new procedure using a non-suture cuff technique. 
In recent years, we have performed more than 300 pancreas transplantations in mice with an overall success rate of &gt;90%, a success rate never described before in mouse pancreas transplantation. The backbone of this non-suture cuff technique for graft revascularization consists of two major steps: (I) pulling the recipient vessel over a polyethylene/polyamide cuff and fixing it with a circumferential ligature, and (II) placing the donor vessel over the everted recipient vessel and fixing it with a second circumferential ligature. The resultant continuity of the endothelial layer results in less thrombogenic lesions with high patency rates and, finally, high success rates.
In this model, arterial anastomosis is achieved by pulling the abdominal aorta of the donor graft over the everted common carotid artery of the recipient animal. Venous drainage of the graft is achieved by pulling the portal vein of the graft over the everted external jugular vein of the recipient. This manuscript provides details and crucial steps of the organ recovery and organ implantation procedures, which will allow researchers with microsurgical skills to perform the transplantation successfully in their laboratories.

Among abdominal solid organ transplantation, pancreatic grafts are prone to develop severe ischemia reperfusion injury-associated graft damage, leading eventually to early graft loss. This protocol describes a model of murine pancreas transplantation using a non-suture cuff technique, ideally suited for analyzing these early, deleterious damages.

Fare modeli değiştirilmiş manşet tekniği kullanarak pankreas nakli için

Mouse models have several advantages in transplantation research, including easy handling, a variety of genetically well-defined strains, and the ...

A Pre-Clinical Porcine Model of Orthotopic Heart Transplantation

Fifty-years following the first successful report, cardiac transplantation remains the gold-standard treatment for eligible patients with advanced heart failure. Multiple small-animal models of heart transplantation have been used to study the acute and long-term effects of novel therapies. However, few are tested and demonstrated success in clinical trials. It is of critical importance to evaluate new therapies in a clinically relevant large-animal model for efficient and reliable translation of basic studies&#39;&#160;findings. Here, we describe a pre-clinical large-animal (porcine) model of orthotopic heart transplantation that has been firmly established and previously used to investigate novel cardioprotective strategies. This procedure focuses on acute ischemia-reperfusion injury and is a reliable method to investigate novel interventions which have been tested and validated in smaller experimental models, such as the murine model. We demonstrate its usefulness in assessing cardiac performance during the early post-transplantation period and other potential possibilities enabled by the model.

Here, we describe a pre-clinical large-animal (porcine) model of orthotopic heart transplantation that has been firmly established and utilized to investigate novel cardioprotective strategies.

Ortootopik kalp transplantasyonunun klinik öncesi porcine modeli

Fifty-years following the first successful report, cardiac transplantation remains the gold-standard treatment for eligible patients with advanced heart ...

Murine Cervical Aortic Transplantation Model using a Modified Non-Suture Cuff Technique

With the introduction of powerful immunosuppressive protocols, distinct advances are possible in the prevention and therapy of acute rejection episodes. However, only minor improvement in the long-term results of transplanted solid organs could be observed over the past decades. In this context, chronic allograft vasculopathy (CAV) still represents the leading cause of late organ failure in cardiac, renal and pulmonary transplantation.
Thus far, the underlying pathogenesis of CAV development remains unclear, explaining why effective treatment strategies are presently missing and emphasizing a need for relevant experimental models in order to study the underlying pathophysiology leading to CAV formation. The following protocol describes a murine heterotopic cervical aortic transplantation model using a modified non-suture cuff technique. In this technique, a segment of the thoracic aorta is interpositioned in the right common carotid artery. With the use of the non-suture cuff technique, an easy to learn and reproducible model can be established, minimizing the possible heterogeneity of sutured vascular micro anastomoses.

Here, we present a protocol of heterotopic aortic transplantation in mice using the non-suture cuff technique in a cervical murine model. This model can be used to study the underlying pathology of chronic allograft vasculopathy (CAV) and can help evaluate new therapeutic agents in order to prevent its formation.

Modifiye Dikişsiz Manşet Tekniği Kullanılarak Murine Servikal Aort Transplantasyon Modeli

With the introduction of powerful immunosuppressive protocols, distinct advances are possible in the prevention and therapy of acute rejection episodes. ...

A Simplified Model for Heterotopic Heart Valve Transplantation in Rodents

There is an urgent clinical need for heart valve replacements that can grow in children. Heart valve transplantation is proposed as a new type of transplant with the potential to deliver durable heart valves capable of somatic growth with no requirement for anticoagulation. However, the immunobiology of heart valve transplants remains unexplored, highlighting the need for animal models to study this new type of transplant. Previous rat models for heterotopic aortic valve transplantation into the abdominal aorta have been described, though they are technically challenging and costly. For addressing this challenge, a renal subcapsular transplant model was developed in rodents as a practical and more straightforward method for studying heart valve transplant immunobiology. In this model, a single aortic valve leaflet is harvested and inserted into the renal subcapsular space. The kidney is easily accessible, and the transplanted tissue is securely contained in a subcapsular space that is well vascularized and can accommodate a variety of tissue sizes. Furthermore, because a single rat can provide three donor aortic leaflets and a single kidney can provide multiple sites for transplanted tissue, fewer rats are required for a given study. Here, the transplantation technique is described, providing a significant step forward in studying the transplant immunology of heart valve transplantation.

This protocol describes a simple and efficient method for the transplantation of aortic valve leaflets under the renal capsule to allow for the study of alloreactivity of heart valves.