This work was supported by the Interdisciplinary Center for Clinical Research (IZKF) M&#252;nster (SEED 03/12, SB), Deutsche Forschungsgemeinschaft (SFB TR128, TP B6 to S.G.M. ME3283/2-1 to S.G.M.) and by Innovative Medizinische Forschung, M&#252;nster (I-BI111316, SB and SGM).

Fareler kullanan tüm deneyler ilgili kurumsal hayvan bakımı ve kullanımı komitesi kurallarına uygun olarak yapılmalıdır. Fare Deneyler için 1. Genel Yorumlar <ol><li> Patojen içermeyen koşullar altında fareler tutun ve onlara yiyecek ve su ad libitum erişim sağlar. NOT: immünolojik desenler yaş ve cinsiyet ile değişebilir çünkü deney gruplarında yaş ve cinsiyet uyumlu fareler kullanmak önemlidir. </li></ol> 2. hazırlanması ve Aktivasyon OVA-spesıfik CD8 + T hücreleri (OT-I) <ol><li> Beyin dilimleri hazırlama 5 gün, aşağıda tarif edildiği gibi OT-I T hücrelerinin uyarılmasını yapın. Not: 5 x 10 5 aktive efektör CD8 + OT-I T hücreleri, (CD8 + T hücreleri, MHC-I-molekülleri bağlamında sadece OVA257-264 peptidi tanıyan transgenik farelerin) dilim başına gerekli olan konsantrasyonu 5 x 10 5, deney seçildihücreler aynı zamanda, dilim içine göç ve başlar bir kez iyi bir hücre-hücre etkileşimi lehine optimum olarak müttefik, hücrelerin miktarı tek hücre 8,18 sayma sağlayan bir aşırı reaksiyonunu uyarmak için çok yüksek değil. </li><li> OT-I T hücrelerinin kültürlenmesi için ortam hazırlar. % 5 fetal dana serumu (FCS), 10 mM HEPES, 2 mM L-glutamin, 50 uM 2-merkaptoetanol,% 1 esansiyel olmayan amino asitler ve 25 ug / ml gentamisin 500 mi DMEM Supplement. Kullanılana kadar 4 ° C &#39;de orta saklayın. </li><li> Referans 16&#39;daki gibi OT-I transgenik farelerin dalak çıkarın. Hazırlanan ortama aktarabilirsiniz. </li><li> 5 dakika, 4 ° C 300 xg&#39;de 70 mikron süzgeç ve santrifüj süspansiyon yoluyla dalak ezme yoluyla tek bir hücre süspansiyonu oluşturun. </li><li> Kırmızı kan hücreleri lize etmek için 5 dakika boyunca 5 mi amonyum klorür-potasyum (ACK), tampon (150 mM NH4CI, 10 mM KHCO 3, 0.1 mM EDTA, pH 7.3) ile bir hücre süspansiyonu inkübe edin. </li><li> Kuluçkalamadan DurTekrar 10 ml ortam santrifüj o ile ilgili yukarıda tarif edildiği gibi. </li><li> Orta hücre süspansiyonu seyreltin ve sayıları hesaplamak. Plaka 3 x 10 7 hücre yoğunluğunda hücre / kuyuda 12-kuyu plaka ve birinci bunları inkübasyon tarafından OVA 257 - 264 (SIINFEKL; 1 nM) ve IL-2 (IL-2), (500 lU / ml) 5 gün. </li><li> 4 gün sonra, 500 IU / ml yoğunlukta yeniden IL-2 ilave edin. </li><li> Uyarımı takiben, imalatçının talimatları izlenerek bir negatif seçim göre fare CD8 + T hücresi izolasyon kiti kullanılarak hücre süspansiyonundan OT-I T hücrelerinin saflaştırılması. Arıtma CD4, CD11b, CD11c, CD19, CD45R (B220), CD49b (DX5), CD105, MHC Sınıf II Ter-119 ve TCRγ karşı bir antikor kokteyli olmayan tüm CD8 + hücrelerinin azalmasına dayanmaktadır / δ daha sonra saflaştırılmış CD8 + T hücrelerinin, yıkama için manyetik sütun kullanarak atılır olan. NOT: Bu procedu Kritik adımlaryeniden üretici tarafından gösterilir: bu topakların bulunması yıkama aşaması esnasında sütun bloke çünkü reaksiyon sadece tek hücreler içeren çok önemlidir; Byotin kokteyl ilave edilmeden önce hücre sayımı örnek saflık derecesi için önemlidir ve işlem spesifik antikor bağlantıları en aza indirmek için, buz üzerinde yapılmalıdır. </li></ol> OT-I T-hücreleri ile Akut Beyin Dilimleri ve Co-kültür 3. Hazırlık <ol><li> Akut beyin hazırlanmasından önce 19, 200 mM sakaroz, 20 mM PIPES, 2.5 mM KCI, 1.25 mM NaH 2 PO 4, 10 mM MgSO 4, 0.5 CaCl2 ve 10 mM dekstroz ile placedine buz soğukluğunda fizyolojik yemek tuzu çözeltisi hazırlamak dilimler ve yapay serebrospinal akışkan (ACSF) 125 mM NaCI, 2.5 KCl, ile 1.25 NaH 2 PO 4, 24 mM NaHCO 3, 2 mM MgSO 4, 10 mM dekstroz, 2 mM CaCl2,. Usin ile 7,35 her çözeltinin pH ayarlamag NaOH. ACSF pH değerinin ayarlanması önce çözelti,% 95 O2 ve% 5 CO2 karışımı ile kabarcıklar halinde olmalıdır. </li><li> Çözüm Öncesi serin. </li><li> 8 uyutmak - (10 haftalık fareler gibi, C57Bl% 5.0 izofluran,% 5.0 halotan veya bekleyin ip üzerinden 100 mg / kg ketamin ve 10 mg / kg vücut ağırlığı ksilazini enjekte kullanarak kontrol veya inhalasyon ile transgenik ODC-OVA fareler 17 gibi / 6 anestezi. anestezi düzeyini değerlendirmek ve bir kerede onları başını kesmek için bir ayak tutam kullanmak ve kurumsal hayvan bakımı göre fareler Euthanize ve ötenazi için komite kriterlerini kullanabilirsiniz. </li><li> Ventral pozisyonda fare koyun. Dezenfekte ve sagittal saç derisini kesti. , Sagitally kranial kemik açın, bir kepçe yardımı ile hızlı bir şekilde beyin kaldırmak ve tutkal kullanarak bir vibratome plaka üzerine sabitleyin. </li><li> Hazırlanan placedine buz soğukluğunda fizyolojik yemek tuzu çözeltisi ile plaka doldurun. </li><li> Vibratome ile 300 mikron koronal dilim kesin. NOT: Bu prosedür biliyorumn, en az 8 saat, 19-21 arasında bir zaman aralığı içinde işlevsel hücresel çalışmalar için uygun olan canlı sağlam doku örneklerinin elde edilir. Nitekim, zaten 6 saat sonra başlayan bu süre, sonra, hazırlık azaltılmış kalite, aksiyon potansiyeli üretimi ve yayılması gibi temel ve fonksiyonel özelliklerinde yani değişiklikleri gösterir. </li><li> Kesit sonra ACSF ile dolu bir 12 oyuklu plakanın her bir oyuğuna, hemen bir dilim aktarın. </li><li> Dilim başına dikkatli bir şekilde 5 x 10 5 OT-I T hücrelerinin ekleyin. </li><li> Bir kuluçka makinesi içinde en fazla 8 saat (37 ° C,% 5 CO2) için dilim inkübe edin. </li><li> Kuluçka dönemi, hasat dilimleri ve sonra Ekim bileşik Doku Tek kullanarak onları gömmek ve sıvı azot onları dondurmak. Değerlendirilmesi, örneğin, daha fazla histolojik çalışmalar için -20 ° C &#39;de nöronal yapı (örneğin, kullanılarak anti-MAPII ya da anti-sinaptofisin antikorlar) ya da apoptozu (saklayın örneğin, bir anti-Kaspaz-3 antikorları, anti-neun anti kullanarakorganları), standart prosedürlere göre yöntem. </li></ol>

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J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Subpial+demyelination+in+the+cerebral+cortex+of+multiple+sclerosis+patients.">Subpial demyelination in the cerebral cortex of multiple sclerosis patients.</a> J Neuropathol Exp Neurol. 62 (7), 723-732 (2003).</li><li>Babbe, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Clonal+expansions+of+CD8(+)+T+cells+dominate+the+T+cell+infiltrate+in+active+multiple+sclerosis+lesions+as+shown+by+micromanipulation+and+single+cell+polymerase+chain+reaction.">Clonal expansions of CD8(+) T cells dominate the T cell infiltrate in active multiple sclerosis lesions as shown by micromanipulation and single cell polymerase chain reaction.</a> J Exp Med. 192 (3), 393-404 (2000).</li><li>Junker, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multiple+sclerosis:+T-cell+receptor+expression+in+distinct+brain+regions.">Multiple sclerosis: T-cell receptor expression in distinct brain regions.</a> Brain. 130 (11), 2789-2799 (2007).</li><li>Skulina, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multiple+sclerosis:+brain-infiltrating+CD8++T+cells+persist+as+clonal+expansions+in+the+cerebrospinal+fluid+and+blood.">Multiple sclerosis: brain-infiltrating CD8+ T cells persist as clonal expansions in the cerebrospinal fluid and blood.</a> Proc Natl Acad Sci U S A. 101 (8), 2428-2433 (2004).</li><li>Friese, M. 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S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+pathogenic+role+for+myelin-specific+CD8(+)+T+cells+in+a+model+for+multiple+sclerosis.">A pathogenic role for myelin-specific CD8(+) T cells in a model for multiple sclerosis.</a> J Exp Med. 194 (5), 669-676 (2001).</li><li>McPherson, S. W., Heuss, N. D., Roehrich, H., Gregerson, D. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bystander+killing+of+neurons+by+cytotoxic+T+cells+specific+for+a+glial+antigen.">Bystander killing of neurons by cytotoxic T cells specific for a glial antigen.</a> Glia. 53 (5), 457-466 (2006).</li><li>Hogquist, K. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=T+cell+receptor+antagonist+peptides+induce+positive+selection.">T cell receptor antagonist peptides induce positive selection.</a> Cell. 76 (1), 17-27 (1994).</li><li>Cao, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Induction+of+experimental+autoimmune+encephalomyelitis+in+transgenic+mice+expressing+ovalbumin+in+oligodendrocytes.">Induction of experimental autoimmune encephalomyelitis in transgenic mice expressing ovalbumin in oligodendrocytes.</a> Eur J Immunol. 36 (1), 207-215 (2006).</li><li>G&#246;bel, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=CD4(+)+CD25(+)+FoxP3(+)+regulatory+T+cells+suppress+cytotoxicity+of+CD8(+)+effector+T+cells:+implications+for+their+capacity+to+limit+inflammatory+central+nervous+system+damage+at+the+parenchymal+level.">CD4(+) CD25(+) FoxP3(+) regulatory T cells suppress cytotoxicity of CD8(+) effector T cells: implications for their capacity to limit inflammatory central nervous system damage at the parenchymal level.</a> J Neuroinflammation. 9, 41 (2012).</li><li>Edwards, F. 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The authors declare that they have no competing financial interests.

Farklı hayvan modelleri MS gibi inflamatuar demiyelinizan hastalıklarının patolojik özellikleri ele son on yıl içinde tarif edilmiştir. In vivo fare hem de sıçan modelleri yaygın bir hastalık patofizyolojik özelliklerini taklit etmek için kullanılır, yani, demiyelinasyon ve remiyelinizasyon işlemlerinin sonuçlarının analizi ve inflamasyon ve nörodejenerasyonda içiçe bölüm. Bununla birlikte, sadece bir ex vivo yaklaşım tam altında yatan mekanizmaları incelemek için izin ver...

Death of oligodendrocytes and destruction of the myelin sheath accompanied by loss of neurons and axons are typical pathological findings in grey matter lesions in individuals suffering from multiple sclerosis (MS)1,2. Cortical lesions can be divided so far in three different subtypes2: subpial, intracortical and leukocortical lesions. In comparison to white matter plaques, infiltrates are characterized by a predominance of CD8+ T-cells, suggesting their possible decisive role in grey matter inflammation3. Furthermore, oligoclonal expansions in blood, cerebrospinal fluid (CSF) and within inflammatory lesions can be found for CD8+ T-cells themselves4-6. 
In line with this, it is assumed that CD8+ T-cells may be specific for different myelin proteins7,8. Indeed, CD8+ T-cells are found near oligodendrocytes and myelin sheaths9,10 that show MHC I expression11 and might therefore be responsible for the loss of the myelin sheath. This process is often seen together with extensive &#8216;&#8216;collateral&#8217;&#8217; neuronal and axonal damage within the central nervous system (CNS) grey matter1,2. In fact, direct and indirect death of oligodendrocytes and neurons is induced by CD8+ T-cells via two different mechanism: (i) cell membrane swelling and rupture due to the formation of cytotoxic granules following the release of perforins and granzymes and (ii) ligation to the Fas receptors or exposition of FasL on their surface8,12,13.
Different types of animal models are established to study the underlying mechanism of the mentioned processes. In this respect, primed CD8+ T-cells specific for autoantigens with induced expression in CNS glial cells, like oligodendrocytes or astrocytes, can be adoptively transferred to analyse &#8216;&#8216;collateral&#8217;&#8217; neuronal and axonal death in grey matter subsequently14,15. To perform such in vivo experiments is a big help to mimic some pathophysiological aspects of MS, however, this approach is not suited to resolve the underlying mechanism and kinetics of axonal damage and neuronal apoptosis.
To overcome these restrictions, an ex vivo approach was established to study the mechanisms and time course of neuronal cell death following a oligondendrocytes-directed CD8+ T-cell attack. Since only oligodendrocytes and therefore myelin sheath production should be targeted by immune cells, MHC class-I-restricted, ovalbumin (OVA)-reactive OT-I Tcells are used16. These cells are subsequently transferred into brain slices obtained from mice selectively expressing OVA in oligodendrocytes (ODC-OVA mice)17.

<table border="0" cellpadding="0" cellspacing="0" width="786">
	<tbody>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Name of Material/ Equipment</td>
			<td style="width:187px;">Company</td>
			<td style="width:119px;">Catalog Number</td>
			<td style="width:264px;">Comments/Description</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">12-Well-plate</td>
			<td style="width:187px;">Corning</td>
			<td style="width:119px;">3513</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">2-Mercaptoethanol</td>
			<td style="width:187px;">Gibco</td>
			<td style="width:119px;">31350-010</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">2-Methylbutan</td>
			<td style="width:187px;">Roth</td>
			<td style="width:119px;">3927.1</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">70 &#181;m strainer</td>
			<td style="width:187px;">Falcon</td>
			<td style="width:119px;">352350</td>
			<td></td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;width:216px;">CaCl2</td>
			<td style="width:187px;">Merck</td>
			<td style="width:119px;">1.02382.0500</td>
			<td>calcium chloride</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;width:216px;">CD8+-isolation kit</td>
			<td style="width:187px;">Miltenyi Biotech</td>
			<td style="width:119px;">130-090-859</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">D(+)-glukose</td>
			<td style="width:187px;">Merck</td>
			<td style="width:119px;">1.08337.1000</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">DMEM</td>
			<td style="width:187px;">Gibco</td>
			<td style="width:119px;">31966-021</td>
			<td>warm in 37 &#176;C water bath before use</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">EDTA</td>
			<td style="width:187px;">Sigma</td>
			<td style="width:119px;">E5134</td>
			<td></td>
		</tr>
		<tr height="26">
			<td height="26" style="height:26px;width:216px;">FCS</td>
			<td style="width:187px;">PAA Laboratories</td>
			<td style="width:119px;">A15-151</td>
			<td>fetal calve serum</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">gentamicin</td>
			<td style="width:187px;">Gibco</td>
			<td style="width:119px;">15750-060</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">HEPES 1M</td>
			<td style="width:187px;">Gibco</td>
			<td style="width:119px;">15630-050</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">IL-2</td>
			<td style="width:187px;">Peprotech</td>
			<td style="width:119px;">212-12</td>
			<td></td>
		</tr>
		<tr height="26">
			<td height="26" style="height:26px;width:216px;">Isofluran</td>
			<td style="width:187px;">Abbott</td>
			<td style="width:119px;">05260-05</td>
			<td></td>
		</tr>
		<tr height="26">
			<td height="26" style="height:26px;width:216px;">KCl</td>
			<td style="width:187px;">Merck</td>
			<td style="width:119px;">1.04933.0500</td>
			<td>potassium chloride</td>
		</tr>
		<tr height="26">
			<td height="26" style="height:26px;width:216px;">KHCO3</td>
			<td style="width:187px;">Sigma</td>
			<td style="width:119px;">P9144</td>
			<td>potassium hydrogen carbonate</td>
		</tr>
		<tr height="26">
			<td height="26" style="height:26px;width:216px;">L-Glutamine</td>
			<td style="width:187px;">Gibco</td>
			<td style="width:119px;">35050-038</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">MgSO4</td>
			<td style="width:187px;">Merck</td>
			<td style="width:119px;">1.05886.0500</td>
			<td>magnesium sulfate</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">NaCl</td>
			<td style="width:187px;">Sigma</td>
			<td style="width:119px;">31434</td>
			<td>sodium chloride</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">NaH2PO4 * H2O</td>
			<td style="width:187px;">Merck</td>
			<td style="width:119px;">1.06346.0500</td>
			<td>sodium hydrogen phosphate</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">NaHCO3</td>
			<td style="width:187px;">Merck</td>
			<td style="width:119px;">1.06329.0500</td>
			<td>sodium hydrogen carbonate</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">NaOH</td>
			<td style="width:187px;">Merck</td>
			<td style="width:119px;">1.09137.1000</td>
			<td>sodium hydroxide</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:216px;">NH4Cl</td>
			<td style="width:187px;">Sigma</td>
			<td style="width:119px;">213330</td>
			<td>ammonium chloride</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">Non Essential Amino Acid</td>
			<td style="width:187px;">Gibco</td>
			<td style="width:119px;">11140-050</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">OVA (257-264)</td>
			<td style="width:187px;">Genscript</td>
			<td style="width:119px;">RP10611</td>
			<td>ovalbumin</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">PIPES</td>
			<td style="width:187px;">Sigma</td>
			<td style="width:119px;">P6757</td>
			<td></td>
		</tr>
		<tr height="26">
			<td height="26" style="height:26px;width:216px;">Sukrose</td>
			<td style="width:187px;">Merck</td>
			<td style="width:119px;">1.07687.1000</td>
			<td></td>
		</tr>
		<tr height="26">
			<td height="26" style="height:26px;width:216px;">Tissue-Tek OCT</td>
			<td style="width:187px;">Sakura</td>
			<td style="width:119px;">4583</td>
			<td></td>
		</tr>
	</tbody>
</table>

an ex vivo model of an oligodendrocyte-directed t-cell attack in acute brain slices

Death of oligodendrocytes accompanied by destruction of neurons and axons are typical histopathological findings in cortical and subcortical grey matter lesions in inflammatory demyelinating disorders like multiple sclerosis (MS). In these disorders, mainly CD8+ T-cells of putative specificity for myelin- and oligodendrocyte-related antigens are found, so that neuronal apoptosis in grey matter lesions may be a collateral effect of these cells. Different types of animal models are established to study the underlying mechanisms of the mentioned pathophysiological processes. However, although they mimic some aspects of MS, it is impossible to dissect the exact mechanism and time course of &#8216;&#8216;collateral&#8217;&#8217; neuronal cell death. To address this course, here we show a protocol to study the mechanisms and time response of neuronal damage following an oligodendrocyte-directed CD8+ T cell attack. To target only the myelin sheath and the oligodendrocytes, in vitro activated oligodendrocyte-specific CD8+ T-cells are transferred into acutely isolated brain slices. After a defined incubation period, myelin and neuronal damage can be analysed in different regions of interest. Potential applications and limitations of this model will be discussed.

Oligodendrosit yönelik CD8 + T hücreleri ile beyin dilimlerindeki inkübasyondan sonra, oligodendrositler olarak nöronlar apoptoz (Şekil 2A ve 1C, sırasıyla) tabi tutulur. Apoptoz histolojik bulgular (örneğin, Kaspaz-3, Tünel) ilk inkübasyon 3 saat sonra tespit edilebilir. Kuluçka dönemi hazırlanması ve tekrarlanabilir sonuçlar iyi kalitesini garanti etmek için uzun 8 saat daha olmamalıdır. Apoptotik hücreler tüm miyelinli alanlarda üstünlüğü ile dilim üzeri...

Watch this Scientific Journal Video about An Ex vivo Model of an Oligodendrocyte-directed T-Cell Attack in Acute Brain Slices at JoVE.com

An Ex vivo Model of an Oligodendrocyte-directed T-Cell Attack in Acute Brain Slices

To address mechanisms of demyelination and neuronal apoptosis in cortical lesions of inflammatory demyelinating disorders, different animal models are used. We here describe an ex vivo approach by using oligodendrocyte-specific CD8+ T-cells on brain slices, resulting in oligodendroglial and neuronal death. Potential applications and limitations of the model are discussed.

Bir<em&gt; Ex vivo</em&gt; Akut Beyin Dilimleri bir Oligodendrosit yönelik T-Hücre Saldırı Modeli

Death of oligodendrocytes accompanied by destruction of neurons and axons are typical histopathological findings in cortical and subcortical grey matter ...

an-ex-vivo-model-an-oligodendrocyte-directed-t-cell-attack-acute

Research

JoVE Journal

Immunology and Infection

An Ex vivo Model of an Oligodendrocyte-directed T-Cell Attack in Acute Brain Slices

6.8K Görüntüleme.  University of Münster. To address mechanisms of demyelination and neuronal apoptosis in cortical lesions of inflammatory demyelinating disorders, different animal models are used. We here describe an ex vivo approach by using oligodendrocyte-specific CD8+ T-cells on brain slices, resulting in oligodendroglial and neuronal death. Potential applications and limitations of the model are discussed.