Express and Purify Proteins with Identified Domain Boundaries
2:38
Characterize Protein Sample to Identify Those with Advantageous Properties
4:16
Producing High Quality Crystals of the Coiled-coil SD2 Domain from Shroom
8:43
Results: Evaluation of Expression, Purification, Characterization, and Crystallization of Candidate Coiled-coil Proteins
9:49
Conclusion
Transkript
The overall goal of this procedure is to rapidly identify and test possible domain boundaries within large coiled-coil proteins to improve the likelihood of finding an intrinsic fragment that will crystallize. This method can help researchers stre
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We describe a framework incorporating straightforward biochemical and computational analysis to guide the characterization and crystallization of large coiled-coil domains. This framework can be adapted for globular proteins or extended to incorporate a variety of high-throughput techniques.