Preparations and Culture of RBL Cells for Live Cell Microscopy
1:52
Live Cell Microscopy of Exocytosis
3:43
Results: FITC-dextran May Act as a Reporter for Regulated Exocytosis
4:17
Conclusion
Transkript
This method can help answer key questions in the research of regulated exocytosis, by allowing researchers to distinguish between the different modes of exocytosis in the living cell. The main advantage of this technique is that it is simple and r
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Here we detail a method for live cell imaging of regulated exocytosis. This method utilizes FITC-dextran, which accumulates in lysosome-related organelles, as a reporter. This simple method also allows distinguishing between different modes of regulated exocytosis in cells that are difficult to manipulate genetically.