This method can help clarify the spinal mechanisms underlying sensory transmission, nociceptive regulation and chronic pain or ache development The main advantage of this technique is that it can permit ideal neuronal preservation and it can mimic
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Here, we describe the essential steps for whole-cell patch-clamp recordings made from substantia gelatinosa (SG) neurons in the in vitro spinal cord slice. This method allows the intrinsic membrane properties, synaptic transmission and morphological characterization of SG neurons to be studied.