13C, 15N-Labeled Aspergillus fumigatus Liquid and Growth Media Preparation
3:03
Fungal Materials Growth
4:21
A. fumigatus Preparation for Solid-State Nuclear Magnetic Resonance (NMR) and Dynamic Nuclear Polarization (DNP) Studies
6:47
Plant Biomass Preparation for DNP Analysis
7:49
Results: Fungal Cell Wall Structure Characterization by Solid-State NMR
9:02
Conclusion
Transkript
This protocol can be used to prepare fungal and plant materials for solid-state nuclear magnetic resonance, or NMR, and dynamic nuclear polarization, or DNP, experiments for complex biosystem characterization. This technique allows us to investiga
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A protocol for preparing 13C,15N-labeled fungal and plant samples for multidimensional solid-state NMR spectroscopy and dynamic nuclear polarization (DNP) investigations is presented.