Results: Survival and Efficiency Rate of RNAi and CRISPR/Cas9 via Pupal and Adult Injections
7:52
Conclusion
Transkript
Research on non-canonical insect model, like parasitoid wasp, is limited because embryonic microinjection is very challenging. Our method bypasses this problem, enabling site-specific and heritable germline mutations. Injecting pupae or adult fema
Bu içeriğe erişmek için giriş yapın veya ücretsiz denemenizi başlatın
Here, we describe methods for efficient pupal and adult injections in Nasonia vitripennis as accessible alternatives to embryo microinjection, enabling functional analysis of genes of interest using either RNA-silencing via RNA interference (RNAi) or gene knockout via CRISPR/Cas9 genome editing.