mGluR2 Expression with Incorporated Unnatural Amino Acid, Fluorescent Labeling, and Extraction
5:13
Single-Molecule Flow Chamber Assembly and Functionalization
6:23
Microscope Setup and smFRET Data Acquisition
7:47
Data Analysis
8:39
Results: Analyzing Conformational Dynamics of Membrane Receptors Proteins
10:11
Conclusion
Transkript
Here, we used single-molecule FRET, and site-specific protein labeling via a natural amino acid incorporation to study the conformational dynamics of mGluR2. This allows to study the protein dynamics without perturbing protein structure. Atomic st
Bu içeriğe erişmek için giriş yapın veya ücretsiz denemenizi başlatın
This study presents a detailed procedure to perform single-molecule fluorescence resonance energy transfer (smFRET) experiments on G protein-coupled receptors (GPCRs) using site-specific labeling via unnatural amino acid (UAA) incorporation. The protocol provides a step-by-step guide for smFRET sample preparation, experiments, and data analysis.