Kabul edilmiş DTH indüksiyonu <a href="/index/Details.stp?ID=325">İndüksiyon ve İzleme</a> Lütfen vallahi makalesine bakın: <a href="/index/Details.stp?ID=325">Sıçanlarda Kabul edilmiş Gecikmeli-Tipi aşırı duyarlılık</a> Christine Beeton George K. Chandy Fizyoloji ve Biyofizik Bölümü, University of California, Irvine J. görüntülenmiştir Deneyler, Sayı 8 Burada, bu protokol fagositik antijen-sunan hücreler görünüm için izin etiketsiz antijen ile 1:1 oranında antijen (ovalbumin) Texas-Kırmızı konjuge kullanarak modifiye ettiler. Kulak Doku Hasat <ol><li> İstediğiniz zaman noktada onaylanmış hayvan protokolde belirtilen usul ile sıçan Euthanize. Burada, aşırı dozda anestezi, izofluran kullanın. Bu, iyi havalandırılmış bir kaput içinde bulunan kapalı bir kap içinde yapılır. Hayvan torasik kavite açılması ve kalp kesim ya dekapitasyon ölü olduğundan emin olun. </li><li> Büyük makas kullanarak hayvanın vücuduna mümkün olduğu kadar yakın tek bir kesim, (Şekil 1) kulak çıkarın. <img src="/files/ftp_upload/old/907_2008_8_13_0/Figure_1.jpg" alt="Figure_1.jpg" /> Şekil 1. Rat kulak kaldırılması </li><li> Içeren bir buz 1x PBS veya RPMI-1640 ~ 10 ml 15 ml konik tüp kulak yerleştirin. Buz üzerinde doku örnekleri doku görüntüleme hazırlamak için hazır olana kadar tutun. </li><li> Mümkün olduğunca çabuk Görüntü doku, hasat ve anında görüntülü doku göre 1 ila 2 saat buz üzerinde doku, hücre hareketi üzerinde belirgin bir etkisi olduğunu, ancak, bulabilirsiniz. </li></ol> Görüntüleme için Doku Hazırlama Epidermal ve dermal Katman I. Kaldırma * Not: derin dermal tabaka sağlam tutulmalıdır. Eğer doğru yapılırsa büyük kan damarları kalacaktır ve kulak kıkırdak maruz kalmayacaktır. Herhangi bir inflamasyon (kontrol koşulları) var, özellikle bu zor bir tekniktir ve bir diseksiyon mikroskop kullanılması yararlı olur. <ol><li> 10 cm doku kültürü çanak konik tüp doku ve medya yerleştirin. </li><li> Eldivenli eller, dorsal yüzü yukarı bakacak şekilde (Şekil 2) kulak kulak (distal) ucuna hafifçe sıçan kulak tutun ile. <img src="/files/ftp_upload/old/907_2008_8_13_1/Figure_2.jpg" alt="Figure_2.jpg" /> Şekil 2. Kulak keserek kürk </li><li> Kulak üst, derin dermis dermis ayrı ucu kapalı Dumont # 5 makas kullanırken sabit doku başparmak ile. Alternatif olarak, kulak kenarı deri / derin dermis bir çıkıntı varsa, bu forseps ile kavradı ve yavaşça altındaki doku (Şekil 3) ayrı çekti. <img src="/files/ftp_upload/old/907_2008_8_13_2/Figure_3.jpg" alt="Figure_3.jpg" /> Şekil 3. Üst dermis kaldırılması </li><li> Bir kez cilt küçük bir alanda alternatif hafifçe kaldırın ve yerinden deri soyulabilir, dermal katmanlar ve forseps arasında kesmek için makas kullanarak ayırma adımları tekrarlayın, altta yatan doku ayrılmış oldu. </li><li> 5 x 5 mm veya kulak doku antijen enjeksiyonu ilk site (Şekil 4) en az 3 mm daha geniş bir alana Açığa. <img src="/files/ftp_upload/old/907_2008_8_13_3/Figure_4.jpg" alt="Figure_4.jpg" /> Şekil 4. Görüntüleme için maruz Doku </li></ol> II. Görüntüleme Sahne Doku güvenliğini <ol><li> Plastik bir kapak kayma hazırlanan doku biraz daha büyük bir boyuta kesin. </li><li> 3M Vetbond ™ slaytta doku güvenli yapıştırıcı ince bir tabaka halinde sürün. </li><li> Hazırlanan doku (görüntü muhtemel değildir) köşesinde tutun, medya kaldırmak ve dab doku, lens kağıt veya Kym üzerinde alt aşırı medya kaldırmak için silin. </li><li> Tutkal kapalı slayt (ıslak mendil hemen Vetbond kürleri) doku kenarına dokunun. (Şekil 5) slayt dokunmak için son olan diğer ucunu nazikçe, düz doku germek <img src="/files/ftp_upload/old/907_2008_8_13_4/Figure_5.jpg" alt="Figure_5.jpg" /> Şekil 5. Kayma kapsayacak şekilde doku Dağı </li><li> Cure Vetbond ™ tersine doku örneği / slayt ve daldırma, doku ve slayt yüzü aşağı ve aşağı yukarı 45 derecelik bir açıda olduğu gibi medya bir rezervuar. Ters bir açıyla Holding doku maruz kalan doku yüzey kür fazla tutkal önlemek için yardımcı olur. Tutkal doku yüzeyine lazer engeller (Şekil 6). <img src="/files/ftp_upload/old/907_2008_8_13_5/Figure_6.jpg" alt="Figure_6.jpg" /> Şekil 6. Vetbond doku glu CureMedyada daldırma e Not: Adımlar 2-5 master için biraz pratik gerektirebilir. Ilk kez denemeden önce atılır veya ekstra doku parçaları ile pratik deneyin. </li><li> Dab üzerine silikon gres slayt altındaki küçük bir miktar. </li><li> Perfüzyon odasında slayt yerleştirin. Perfüzyon medya, 37 ° C akan ve doku eklemeden önce oksijenli olduğundan emin olun. Perfüzyon odasının alt ve plastik kapak kayma arasında silikon gres yağı ile bir mühür slayt kenarlarını hafifçe bastırın. </li></ol> III. İki-Foton Görüntüleme <ol><li> Parlak alan iletilen ışık kullanarak, doku üstüne odaklanır. Tüm ışıkları kapatın. </li><li> Lazer açın, çok foton mikroskop sistemi tarafından gerekli Proje Yönetim Ekipleri. </li><li> Ikinci harmonik üretimi, yüksek emretti lifli proteinler (SHG) (kolajen ve miyozin) ikaz lazer yarım dalga boyunda bir sinyal üretir. Mikroskop &quot;mavi&quot; kanalını kullanarak görüntülenebilmekte 450 nm&#39;de SHG önde gelen 900 nM uyarma kullanın. SHG nesil bir non-lineer (iki-foton) işlemi ve bu nedenle floresan iki foton uyarma benzer bir optik &quot;Kesit&quot; etkisi sağlar. Paralel olarak çalışan fibriller görüntülenmiştir izin vermeyecek SHG tepe verimliliği, fibriller lazer dik oluşur. 1 </li><li> Bol hücre ile iyi bir görüntüleme alanı bulun hücrelerin çoğunluğunun merkezi ve görüntüleme başlar, satın alma alanı ayarlayın. Not: DTH reaksiyonlar, T hücreleri ve diğer hücre infiltrasyonlar sıklıkla, diğer alanlarda hücreler 2 infiltre yoksun ise birlikte konsantre olarak bulunmuştur . Bu nedenle, doku görüntü için en uygun alanı bulmak için arama biraz zaman alabilir. </li><li> Görüntüleme sırasında hücre hızı ve polarite değerlendirerek hücre canlılığı için kontrol edin. Minimal fotoğraf zarar görmesini sağlamak için kabul edilebilir bir görüntü kalitesi sağlayan en düşük lazer gücü kullanın. </li><li> Yeni bir doku hazırlanması gerektiği gibi değiştirin. </li></ol>

<ol>
	<li>Koo, G. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Blockade+of+the+voltage-gated+potassium+channel+Kv1.3+inhibits+immune+responses+in+vivo.">Blockade of the voltage-gated potassium channel Kv1.3 inhibits immune responses in vivo.</a> J Immunol. 158, 5120-5128 (1997).</li><li>Gaga, M., Frew, A. J., Varney, V. A., Kay, A. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Eosinophil+activation+and+T+lymphocyte+infiltration+in+allergen-induced+late+phase+skin+reactions+and+classical+delayed-type+hypersensitivity.">Eosinophil activation and T lymphocyte infiltration in allergen-induced late phase skin reactions and classical delayed-type hypersensitivity.</a> J Immunol. 147, 816-822 (1991).</li><li>Flugel, A., Odoardi, F., Nosov, M., Kawakami, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Autoaggressive+effector+T+cells+in+the+course+of+experimental+autoimmune+encephalomyelitis+visualized+in+the+light+of+two-photon+microscopy.">Autoaggressive effector T cells in the course of experimental autoimmune encephalomyelitis visualized in the light of two-photon microscopy.</a> J Neuroimmunol. 191, 86-97 (2007).</li><li>Kawakami, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Live+imaging+of+effector+cell+trafficking+and+autoantigen+recognition+within+the+unfolding+autoimmune+encephalomyelitis+lesion.">Live imaging of effector cell trafficking and autoantigen recognition within the unfolding autoimmune encephalomyelitis lesion.</a> J Exp Med. 201, 1805-1814 (2005).</li><li>Miller, M. J., Wei, S. H., Parker, I., Cahalan, M. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Two-photon+imaging+of+lymphocyte+motility+and+antigen+response+in+intact+lymph+node.">Two-photon imaging of lymphocyte motility and antigen response in intact lymph node.</a> Science. 296, 1869-1873 (2002).</li><li>Matheu, M. P. B., A, C. G. a. r. c. i. a., Chi, V., Rangaraju, S., Safrina, O., Monaghan, K., Uemura, M. I., Li, D., Pal, S., Monuki, E., Flugel, A., Pennington, M. W., Parker, I., Chandy, G. K., Calahan, M. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+Situ+Imaging+of+Effector/Memory+T+Cells+During+DTH+and+Suppression+by+Kv1.3+Channel+Block.">In Situ Imaging of Effector/Memory T Cells During DTH and Suppression by Kv1.3 Channel Block.</a> Immunity. , (2008).</li></ol>

imaging effector memory t cells in the ear after induction of adoptive dth

Efektör / bellek T lenfositleri - Gecikmiş tip aşırı duyarlılık (DTH), ana oyuncuları CCR7 olan bir bağışıklık reaksiyonu . Burada, uyaran ve sıçan kulakta bir DTH reaksiyon ilerleme kaydetmek için bir yöntem ortaya koymaktadır. Efektör / bellek T hücre yanıtı - Bu sıçan kulak doku hazırlanması CCR7 iki foton görüntüleme için bir gösteri izledi. Efektör / bellek T hücre hattı (Beeton, C J. görüntülenmiştir deneyler, Sayı: 8) - Bir evlat edinen DTH GFP etiketli Ova özel CCR7 intraperitoneal enjeksiyonu ile indüklenen. Hücreler daha sonra 48 saat önce meydan görselleştirme izin Texas-Kırmızı Ova ve Ova konjuge 1:1 karışımlar (Ova-TR) ile serum fizyolojik (kontrol kulak) ve diğer bir kulak enjekte sıçan denge sağlaması için izin verilir yerleşik antijen-sunan hücreler. Biz bir görüntüleme motilite bir immün yanıt sırasında, ikinci harmonik üretimi, kolajen liflerinin görselleştirme ile birlikte derin dermal tabaka içinde hücreler için yararlı doku hazırlanması yöntemi açıklanmaktadır. Kulak doku 5 x 5 mm kareler (biraz daha büyük daha iyidir) oyulmuş ve sonra 37 görüntüleme odasında silikon gres kullanılarak güvenli ve oksijen kabarmış doku kültürü ortamı superfused Vetbond ™ kullanarak plastik kapak bordroları monte ° C .

Watch this Scientific Journal Video about Imaging Effector Memory T cells in the Ear After Induction of Adoptive DTH at JoVE.com

Imaging Effector Memory T cells in the Ear After Induction of Adoptive DTH

Burada uyaran ve sıçan kulak gecikmiş tip aşırı duyarlılık (DTH) reaksiyon ilerleme kaydetmek için bir yöntem gösteriyor. Bu sıçan kulak doku hazırlanması / bellek efektör T hücre yanıtının iki foton görüntüleme için bir gösteri izledi.

Kabul edilmiş DTH indüksiyonu sonra Kulak Görüntüleme Efektör Bellek T hücreleri

Delayed type hypersensitivity (DTH) is an immune reaction in which the main players are CCR7- effector / memory T lymphocytes. Here, we demonstrate a ...

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Research

JoVE Journal

Biology

10.2K Görüntüleme.  University of California, Irvine (UCI). Burada uyaran ve sıçan kulak gecikmiş tip aşırı duyarlılık (DTH) reaksiyon ilerleme kaydetmek için bir yöntem gösteriyor. Bu sıçan kulak doku hazırlanması / bellek efektör T hücre yanıtının iki foton görüntüleme için bir gösteri izledi.

Video: Imaging Effector Memory T cells in the Ear After Induction of Adoptive DTH

Induction and Monitoring of Active Delayed Type Hypersensitivity (DTH) in Rats

Delayed type hypersensitivity (DTH) is an inflammatory reaction mediated by CCR7- effector memory T lymphocytes that infiltrate the site of injection of an antigen against which the immune system has been primed. The inflammatory reaction is characterized by redness and swelling of the site of antigenic challenge. It is a convenient model to determine the in vivo efficacy of immunosuppressants. Cutaneous DTH can be induced either by adoptive transfer of antigen-specific T lymphocytes or by active immunization with an antigen, and subsequent intradermal challenge with the antigen to induce the inflammatory reaction in a given skin area. DTH responses can be induced to various antigens, for example ovalbumin, tuberculin, tetanus toxoid, or keyhole limpet hemocyanin (KLH).
Here we demonstrate how to induce an active DTH reaction in Lewis rats. We will first prepare a water-in-oil emulsion of KLH, our antigen of interest, in complete Freund's adjuvant and inject this emulsion subcutaneously to rats. This will prime the immune system to develop memory T cells directed to KLH. Seven days later we will challenge the rats intradermally on the back with KLH on one side and with ovalbumin, an irrelevant antigen, on the other side. The inflammatory reaction will be visible 16-72 hours later and the red and swollen area will be measured as an indication of DTH severity.

Induction and Monitoring of Active Delayed Type Hypersensitivity DTH in Rats

Delayed type hypersensitivity (DTH) is an inflammatory reaction mediated by CCR7- effector memory T lymphocytes. Here we demonstrate how to induce active DTH in Lewis rats and monitor the inflammatory response.

Sıçanlarda Aktif Gecikmiş tip aşırı duyarlılık (DTH) İndüksiyon ve İzlenmesi

Delayed type hypersensitivity (DTH) is an inflammatory reaction mediated by CCR7- effector memory T lymphocytes that infiltrate the site of injection of ...

Biyoloji

Induction and Monitoring of Adoptive Delayed-Type Hypersensitivity in Rats

Delayed type hypersensitivity (DTH) is an inflammatory reaction mediated by CCR7- effector memory T lymphocytes that infiltrate the site of injection of an antigen against which the immune system has been primed. The inflammatory reaction is characterized by redness and swelling of the site of antigenic challenge. It is a convenient model to determine the in vivo efficacy of immunosuppressants. Cutaneous DTH can be induced either by adoptive transfer of antigen-specific T lymphocytes or by active immunization with an antigen, and subsequent intradermal challenge with the antigen to induce the inflammatory reaction in a given skin area. DTH responses can be induced to various antigens, for example ovalbumin, tuberculin, tetanus toxoid, or keyhole limpet hemocyanin. Such reactions can also be induced against autoantigen, for example to myelin basic protein (MBP) in rats with experimental autoimmune encephalomyelitis induced with MBP, an animal model for multiple sclerosis (1).
Here we demonstrate how to induce an adoptive DTH reaction in Lewis rats. We will first stimulate ovalbumin-specific T cells in vitro and inject these activated cells intraperitoneally to naive rats. After allowing the cells to equilibrate in vivo for 2 days, we will challenge the rats with ovalbumin in the pinna of one ear, while the other ear wil receive saline. The inflammatory reaction will be visible 3-72 hours later and ear thickness will be measured as an indication of DTH severity.

Delayed type hypersensitivity (DTH) is an inflammatory reaction mediated by CCR7- effector memory T (TEM) lymphocytes. Here we demonstrate how to activate antigen-specific TEM cells, induce adoptive DTH in Lewis rats and monitor the inflammatory response.

Sıçanlarda Kabul edilmiş Gecikmeli-Tipi aşırı duyarlılık indüksiyonu ve İzleme

Primary Culture and Plasmid Electroporation of the Murine Organ of Corti. 

In all mammals, the sensory epithelium for audition is located along the spiraling organ of Corti that resides within the conch shaped cochlea of the inner ear (fig 1). Hair cells in the developing cochlea, which are the mechanosensory cells of the auditory system, are aligned in one row of inner hair cells and three (in the base and mid-turns) to four (in the apical turn) rows of outer hair cells that span the length of the organ of Corti. Hair cells transduce sound-induced mechanical vibrations of the basilar membrane into neural impulses that the brain can interpret. Most cases of sensorineural hearing loss are caused by death or dysfunction of cochlear hair cells.
An increasingly essential tool in auditory research is the isolation and in vitro culture of the organ explant 1,2,9. Once isolated, the explants may be utilized in several ways to provide information regarding normative, anomalous, or therapeutic physiology. Gene expression, stereocilia motility, cell and molecular biology, as well as biological approaches for hair cell regeneration are examples of experimental applications of organ of Corti explants.
This protocol describes a method for the isolation and culture of the organ of Corti from neonatal mice. The accompanying video includes stepwise directions for the isolation of the temporal bone from mouse pups, and subsequent isolation of the cochlea, spiral ligament, and organ of Corti. Once isolated, the sensory epithelium can be plated and cultured in vitro in its entirety, or as a further dissected micro-isolate that lacks the spiral limbus and spiral ganglion neurons. Using this method, primary explants can be maintained for 7-10 days. As an example of the utility of this procedure, organ of Corti explants will be electroporated with an exogenous DsRed reporter gene. This method provides an improvement over other published methods because it provides reproducible, unambiguous, and stepwise directions for the isolation, microdissection, and primary culture of the organ of Corti.

Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.

This procedure describes a method for the isolation and culture of the murine organ of Corti with or without the spiral limbus and spiral ganglion neurons. We also demonstrate a method for the expression of an exogenous reporter gene in the organ of Corti explant by electroporation.

İlköğretim Kültür ve Corti murin Organ Plazmid Elektroporasyon.

In all mammals, the sensory epithelium for audition is located along the spiraling organ of Corti that resides within the conch shaped cochlea of the ...

Clonogenic Assay: Adherent Cells

The clonogenic (or colony forming) assay has been established for more than 50 years; the original paper describing the technique was published in 19561. Apart from documenting the method, the initial landmark study generated the first radiation-dose response curve for X-ray irradiated mammalian (HeLa) cells in culture1. Basically, the clonogenic assay enables an assessment of the differences in reproductive viability (capacity of cells to produce progeny; i.e. a single cell to form a colony of 50 or more cells) between control untreated cells and cells that have undergone various treatments such as exposure to ionising radiation, various chemical compounds (e.g. cytotoxic agents) or in other cases genetic manipulation. The assay has become the most widely accepted technique in radiation biology and has been widely used for evaluating the radiation sensitivity of different cell lines. Further, the clonogenic assay is commonly used for monitoring the efficacy of radiation modifying compounds and for determining the effects of cytotoxic agents and other anti-cancer therapeutics on colony forming ability, in different cell lines. A typical clonogenic survival experiment using adherent cells lines involves three distinct components, 1) treatment of the cell monolayer in tissue culture flasks, 2) preparation of single cell suspensions and plating an appropriate number of cells in petri dishes and 3) fixing and staining colonies following a relevant incubation period, which could range from 1-3 weeks, depending on the cell line. Here we demonstrate the general procedure for performing the clonogenic assay with adherent cell lines with the use of an immortalized human keratinocyte cell line (FEP-1811)2. Also, our aims are to describe common features of clonogenic assays including calculation of the plating efficiency and survival fractions after exposure of cells to radiation, and to exemplify modification of radiation-response with the use of a natural antioxidant formulation.

The applicability of the clonogenic assay for evaluating reproductive viability has been established for more than 50 years. Here we demonstrate the general procedure for performing the clonogenic assay with adherent cells.

Clonogenic Testi: yapışkan Hücreler

The clonogenic (or colony forming) assay has been established for more than 50 years; the original paper describing the technique was published in 19561. ...

Imaging of Estrogen Receptor-&alpha; in Rat Pial Arterioles using a Digital Immunofluorescent Microscope

Many of estrogen's effects on vascular reactivity are mediated through interaction with estrogen receptors 1, 2, 3. Although two sub-types exist (estrogen receptor -&alpha; and &beta;),estrogen receptor-&alpha; has been identified in both the smooth muscle and in endothelial cells of pial arterial segments using fluorescent staining combined with confocal laser scanning microscopy 4. Furthermore, ER-&alpha; is located in the nuclei and in the cytoplasm of rat basilar arteries 5. The receptors are abundant and fluoresce brightly, but clear visualization of discrete groups of receptors is difficult likely due to the numbers located in many cell layers of pial vessel segments. Additionally, many reports using immunohistochemical techniques paired with confocal microscopy poorly detail the requirements critical for reproduction of experiments 6. Our purpose for this article is to describe a simple technique to optimize the staining and visualization of ER-&alpha; using cross-sectional slices of pial arterioles obtain from female rat brains. We first perfuse rats with Evans blue dye to easily identify surface pial arteries which we isolate under a dissecting microscope. Use of a cryostat to slice 8 &mu;m cross sections of the arteries allows us to obtain thin vessel sections so that different vessel planes are more clearly visualized. Cutting across the vessel rather than use of a small vessel segment has the advantage of easier viewing of the endothelial and smooth muscle layers. In addition, use of a digital immunofluorescent microscope with extended depth software produces clear images of ten to twelve different vessel planes and is less costly than use of a confocal laser scanning microscope.

Imaging of Estrogen Receptor-&amp;#945; in Rat Pial Arterioles using a Digital Immunofluorescent Microscope

The goal of this article is to demonstrate a method to optimize immunofluorescent detection of estrogen receptor-&#945; (ER&#945;) in rat pial arterial slices using a digital immunofluorescent microscope.

Rat pial arteriollerde Östrojen Reseptör-α Dijital Immunofluorescent Mikroskobu kullanılarak görüntülenmesi

Many of estrogen's effects on vascular reactivity are mediated through interaction with estrogen receptors 1, 2, 3. Although two sub-types exist (estrogen ...

Induction and Analysis of Epithelial to Mesenchymal Transition

Epithelial to mesenchymal transition (EMT) is essential for proper morphogenesis during development. Misregulation of this process has been implicated as a key event in fibrosis and the progression of carcinomas to a metastatic state. Understanding the processes that underlie EMT is imperative for the early diagnosis and clinical control of these disease states. Reliable induction of EMT in vitro is a useful tool for drug discovery as well as to identify common gene expression signatures for diagnostic purposes. Here we demonstrate a straightforward method for the induction of EMT in a variety of cell types. Methods for the analysis of cells pre- and post-EMT induction by immunocytochemistry are also included. Additionally, we demonstrate the effectiveness of this method through antibody-based array analysis and migration/invasion assays.

A straightforward method is described for the induction of epithelial to mesenchymal transition (EMT) in a variety of cell types. Methods for the analysis of cells in EMT states by immunocytochemistry are included.

Mezenkimal Geçiş indüksiyon ve Epitel Analizi

Epithelial  to mesenchymal transition (EMT) is essential for proper morphogenesis during  development. Misregulation of this  process has been implicated ...

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Cytosolic Ca2+ ions regulate numerous aspects of cellular activity in almost all cell types, controlling processes as wide-ranging as gene transcription, electrical excitability and cell proliferation. The diversity and specificity of Ca2+ signaling derives from mechanisms by which Ca2+ signals are generated to act over different time and spatial scales, ranging from cell-wide oscillations and waves occurring over the periods of minutes to local transient Ca2+ microdomains (Ca2+ puffs) lasting milliseconds. Recent advances in electron multiplied CCD (EMCCD) cameras now allow for imaging of local Ca2+ signals with a 128 x 128 pixel spatial resolution at rates of &#62;500 frames sec-1 (fps). This approach is highly parallel and enables the simultaneous monitoring of hundreds of channels or puff sites in a single experiment. However, the vast amounts of data generated (ca. 1 Gb per min) render visual identification and analysis of local Ca2+ events impracticable. Here we describe and demonstrate the procedures for the acquisition, detection, and analysis of local IP3-mediated Ca2+ signals in intact mammalian cells loaded with Ca2+ indicators using both wide-field epi-fluorescence (WF) and total internal reflection fluorescence (TIRF) microscopy. Furthermore, we describe an algorithm developed within the open-source software environment Python that automates the identification and analysis of these local Ca2+ signals. The algorithm localizes sites of Ca2+ release with sub-pixel resolution; allows user review of data; and outputs time sequences of fluorescence ratio signals together with amplitude and kinetic data in an Excel-compatible table.

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Here we present techniques for imaging local IP3-mediated Ca2+ events using fluorescence microscopy in intact mammalian cells loaded with Ca2+ indicators together with an algorithm that automates identification and analysis of these events.

Görüntüleme Yerel Ca<sup&gt; 2+</sup&gt; Kültürlenmiş memeli hücrelerinde sinyaller

Cytosolic Ca2+ ions regulate numerous aspects of cellular activity in almost all cell types, controlling processes as wide-ranging as gene transcription, ...

High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells

The physiological process by which new vasculature forms from existing vasculature requires specific signaling events that trigger morphological changes within individual endothelial cells (ECs). These processes are critical for homeostatic maintenance such as wound healing, and are also crucial in promoting tumor growth and metastasis. EC morphology is defined by the organization of the cytoskeleton, a tightly regulated system of actin and microtubule (MT) dynamics that is known to control EC branching, polarity and directional migration, essential components of angiogenesis. To study MT dynamics, we used high-resolution fluorescence microscopy coupled with computational image analysis of fluorescently-labeled MT plus-ends to investigate MT growth dynamics and the regulation of EC branching morphology and directional migration. Time-lapse imaging of living Human Umbilical Vein Endothelial Cells (HUVECs) was performed following transfection with fluorescently-labeled MT End Binding protein 3 (EB3) and Mitotic Centromere Associated Kinesin (MCAK)-specific cDNA constructs to evaluate effects on MT dynamics. PlusTipTracker software was used to track EB3-labeled MT plus ends in order to measure MT growth speeds and MT growth lifetimes in time-lapse images. This methodology allows for the study of MT dynamics and the identification of how localized regulation of MT dynamics within sub-cellular regions contributes to the angiogenic processes of EC branching and migration.

Protocols for Human Umbilical Vein Endothelial Cell (HUVEC) culture, transient transfection of fluorescently-labeled markers of microtubule growth, live-cell imaging and automated analysis of interphase microtubule growth dynamics are detailed.

Yüksek çözünürlüklü Time-lapse Görüntüleme ve Yaşayan İnsan Umbilikal Ven Endotelyal Hücrelerinde Mikrotubul Dinamiği Otomatik Analizi

The physiological process by which new vasculature forms from existing vasculature requires specific signaling events that trigger morphological changes ...

A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions

Filopodia are dynamic, finger-like cellular protrusions associated with migration and cell-cell communication. In order to better understand the complex signaling mechanisms underlying filopodial initiation, elongation and subsequent stabilization or retraction, it is crucial to determine the spatio-temporal protein activity in these dynamic structures. To analyze protein function in filopodia, we recently developed a semi-automated tracking algorithm that adapts to filopodial shape-changes, thus allowing parallel analysis of protrusion dynamics and relative protein concentration along the whole filopodial length. Here, we present a detailed step-by-step protocol for optimized cell handling, image acquisition and software analysis. We further provide instructions for the use of optional features during image analysis and data representation, as well as troubleshooting guidelines for all critical steps along the way. Finally, we also include a comparison of the described image analysis software with other programs available for filopodia quantification. Together, the presented protocol provides a framework for accurate analysis of protein dynamics in filopodial protrusions using image analysis software.

We present a software solution for semi-automated tracking of relative protein concentration along the length of dynamic cellular protrusions.

Dinamik Hücresel Protrüzyonlarda Protein Konsantrasyonunun Yazılım Destekli Takibi için Grafiksel Bir Kullanıcı Arayüzü

Filopodia are dynamic, finger-like cellular protrusions associated with migration and cell-cell communication. In order to better understand the complex ...

Efficient Dissection and Culture of Primary Mouse Retinal Pigment Epithelial Cells

Eye disorders affect millions of people worldwide, but the limited availability of human tissues hinders their study. Mouse models are powerful tools to understand the pathophysiology of ocular diseases because of their similarities with human anatomy and physiology. Alterations in the retinal pigment epithelium (RPE), including changes in morphology and function, are common features shared by many ocular disorders. However, successful isolation and culture of primary mouse RPE cells is very challenging. This paper is an updated audiovisual version of the protocol previously published by Fernandez-Godino et al. in 2016 to efficiently isolate and culture primary mouse RPE cells. This method is highly reproducible and results in robust cultures of highly polarized and pigmented RPE monolayers that can be maintained for several weeks on Transwells. This model opens new avenues for the study of the molecular and cellular mechanisms underlying eye diseases. Moreover, it provides a platform to test therapeutic approaches that can be used to treat important eye diseases with unmet medical needs, including inherited retinal disorders and macular degenerations.

This protocol, which was originally reported by Fernandez-Godino et al. in 20161, describes a method to efficiently isolate and culture mouse RPE cells, which form a functional and polarized RPE monolayer within one week on Transwell plates. The procedure takes approximately 3 hours.

Birincil Fare Retina Pigment Epitel Hücrelerinin Verimli Diseksiyonu ve Kültürü

Eye disorders affect millions of people worldwide, but the limited availability of human tissues hinders their study. Mouse models are powerful tools to ...