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Imaging Effector Memory T cells in the Ear After Induction of Adoptive DTH

10.1K Views

09:59 min

August 14th, 2008

August 14th, 2008

10,124 Views

0:01

Title

0:07

Introduction

0:38

Induction of Adoptive DTH and Measuring Inflammation in the Ear

2:54

Removing Dermal Layers from Ear

5:27

Preparing Ear for 2-photon Imaging

7:48

Imaging the T cell Motility in the Ear

9:26

Conclusion

Here we demonstrate a method for inducing and recording the progress of a delayed type-hypersensitivity (DTH) reaction in the rat ear. This is followed by a demonstration of the preparation of rat ear tissue for two-photon imaging of the effector / memory T cell response.

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