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The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation

25.6K Views

•

08:15 min

•

January 6th, 2009

DOI :

10.3791/1026-v

January 6th, 2009

•
1Gene Expression Division, Bio-Rad Laboratories, Inc

Transcript

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Primary Hematopoietic Cell Cultures
Murine Bone Marrow
Electroporation
Plasmid DNA
SiRNA
Gene Pulser MXcell Electroporation System
Gene Pulser Electroporation Buffer
Transfection
Nucleic Acids
Mammalian Cells
Difficult to transfect Cells
Stem Cells
Video Demonstration
Femur
Tibia

Chapters in this video

0:00

Title

0:08

Introduction

0:43

Harvesting Bone Marrow From Femurs and Tibiae

2:07

Establishing Cultures

4:33

Electroporating Cells

7:13

Transfection Results

7:53

Conclusion

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