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Homemade Site Directed Mutagenesis of Whole Plasmids

DOI :

10.3791/1135-v

May 11th, 2009

May 11th, 2009

32,604 Views

1Department of Biology, Johannes Gutenberg-University Mainz, Germany, 2Proteomics division, AlPlanta, Neustadt an der Weinstrasse, Germany

Site directed mutagenesis of whole plasmids is a simple way to create slightly different variations of an original plasmid. Here we demonstrate an easy and cost effective way to introduce base substitutions into a plasmid using standard reagents.

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Site Directed Mutagenesis

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