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Homemade Site Directed Mutagenesis of Whole Plasmids

33.1K Views

07:11 min

May 11th, 2009

DOI :

10.3791/1135-v

May 11th, 2009


Transcript

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Site Directed Mutagenesis

Chapters in this video

0:05

Title

0:10

Introduction

0:47

What We Need

2:47

Thermocycling Reaction

3:29

Thermocycling Conditions

4:09

Gel Check After Reaction

4:28

DpnI Digestion and Transformation

4:58

Transformation

5:30

Screening of Clones

6:47

Conclusion

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