May 11th, 2009
•Site directed mutagenesis of whole plasmids is a simple way to create slightly different variations of an original plasmid. Here we demonstrate an easy and cost effective way to introduce base substitutions into a plasmid using standard reagents.
Related Videos
Principles of Site-Specific Recombinase (SSR) Technology
An Introduction to Worm Lab: from Culturing Worms to Mutagenesis
Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling
Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells
Single Oocyte Bisulfite Mutagenesis
High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay
Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis
A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
Simple Homemade Tools to Handle Fruit Flies—Drosophila melanogaster
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved