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A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli

DOI :

10.3791/57061-v

December 9th, 2017

December 9th, 2017

6,688 Views

1Department of Chemistry and Biochemistry, University of Arkansas, 2Cell and Molecular Biology Program, University of Arkansas, 3Department of Biological Sciences, University of Arkansas, 4Fayetteville High School

Genetic code expansion serves as a powerful tool to study a wide range of biological processes, including protein acetylation. Here we demonstrate a facile protocol to exploit this technique for generating homogeneously acetylated proteins at specific sites in Escherichia coli cells.

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Facile Protocol

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