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Dissection of 6.5 dpc Mouse Embryos

DOI :

10.3791/160-v

February 25th, 2007

February 25th, 2007

37,332 Views

1Massachusetts General Hospital, Harvard Stem Cell Institute, Harvard Medical School

Isolation of postimplantation-stage embryos allows one to study gene patterning and analyze cell-lineage decision making processes during embryonic development, but proper dissection of the early embryo can be challenging. This protocol describes a method for isolating early primitive-streak-stage embryos (~6.5 days post coitum [dpc]).

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Dissection

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