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Protein-protein Interactions Visualized by Bimolecular Fluorescence Complementation in Tobacco Protoplasts and Leaves

DOI :

10.3791/51327-v

March 9th, 2014

March 9th, 2014

20,291 Views

1Department Biologie I, Botanik, Ludwig-Maximilians-Universität, München

Formation of protein complexes in vivo can be visualized by bimolecular fluorescence complementation. Interaction partners are fused to complementary parts of fluorescent tags and transiently expressed in tobacco leaves, resulting in a reconstituted fluorescent signal upon close proximity of the two proteins.

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Protein protein Interactions

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