Analysis of Transgenerational Epigenetic Inheritance in C. elegans Using a Fluorescent Reporter and Chromatin Immunoprecipitation (ChIP)

3.7K Views

10:28 min

May 5th, 2023

DOI :

10.3791/65285-v

May 5th, 2023


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Transgenerational Epigenetic Inheritance

Chapters in this video

0:00

Introduction

1:17

Bleaching and Plating Caenorhabditis elegans P0 Generation Embryos onto RNAi-NGM plates

3:47

Passaging and Scoring the Caenorhabditis elegans P0 Generation for Germline GFP Expression

5:42

Collection of F1 Generation Caenorhabditis elegans for Chromatin Immunoprecipitation (ChIP)

6:38

Formaldehyde Crosslinking of the F1 Generation of Caenorhabditis elegans for ChIP

7:50

Sonication of Formaldehyde-Crosslinked Caenorhabditis elegans and Chromatin Immunoprecipitation

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