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Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae

DOI :

10.3791/57080-v

December 29th, 2017

December 29th, 2017

12,287 Views

1Department of Biological Sciences, University of Maryland

Here, we describe a protocol for chromatin immunoprecipitation of modified histones from the budding yeast Saccharomyces cerevisiae. Immunoprecipitated DNA is subsequently used for quantitative PCR to interrogate the abundance and localization of histone post-translational modifications throughout the genome.

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