Preparation of a Single-Cell Suspension from Mouse Carotid Arteries for Single-Cell Sequencing

2.7K Views

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04:21 min

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January 19th, 2024

DOI :

10.3791/65863-v

January 19th, 2024

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Fengchan Li¹, Zhen Zhu¹, Yun Du¹, Li Zhu¹^,²^,³, Chaojun Tang¹^,²^,³

¹Cyrus Tang Hematology Center, Cyrus Tang Medical Institute, Soochow University, ²Collaborative Innovation Center of Hematology of Jiangsu Province, Soochow University, ³Suzhou Key Laboratory of Thrombosis and Vascular Biology, Soochow University

Transcript

Summary

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Single cell Suspension

Mouse Carotid Arteries

Vascular Tissue Dissociation

Two step Digestion Method

Single cell Sequencing

Carotid Atherosclerosis

Vascular Smooth Muscle Cells

Chapters in this video

0:00

Introduction

1:05

Isolation and Digestion of Mouse Carotid Artery for Single‐Cell Suspension Preparation

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