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Generation of 2D Monolayers from 3D Bovine Ileal Enteroids

347 Views

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04:32 min

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April 5th, 2024

DOI :

10.3791/200825-v

April 5th, 2024

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1Department of Farm Animal Medicine, College of Veterinary Medicine, Midwestern University

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2D Monolayers
3D Bovine Ileal Enteroids
Basement Membrane Extracellular Matrix
BME
Cell Culture
Cell Dissociation
Cell Suspension
Enteroid Fragments
Enteroid Growth Media
FBS Wash Media
Organoid Growth Media
TrypLE Express Enzyme
Trypan Blue Dye Exclusion

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Establishment of a 2D Monolayer from 3D Bovine Ileal Enteroid Culture
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