JoVE Journal

Immunology and Infection

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Direct Observation and Automated Measurement of Stomatal Responses to Pseudomonas syringae pv. tomato DC3000 in Arabidopsis thaliana

1.5K Views

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05:03 min

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February 9th, 2024

DOI :

10.3791/66112-v

February 9th, 2024

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Rikako Hirata*¹, Momoko Takagi*², Yosuke Toda²^,³, Akira Mine¹

¹Graduate School of Agriculture, Kyoto University, ²Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, ³Phytometrics Co., Ltd.

Transcript

Plantal stomata play central roles in adaptation to stress conditions, so we need to measure stomata aperture to understand how plants respond to biotic and abiotic stresses. However, stomata aperture measurement is time-consuming and cumbersome. To measure stomata aperture, we peel the epidermis and observe stomata under a microscope.

Then we manually measure stomata aperture, which takes a long time even for very experienced researchers. We challenged these barriers and developed a tool and a technique that can automatically measure stomata aperture in intact Arabidopsis leaves. So we strongly believe that stomata measuring device and deep-learning algorithm that we developed will facilitate functional analysis of stomata responses to various biotic and abiotic stresses because these technical advances significantly reduce time and human labor required for stomata aperture measurement.

Summary

Here, we present a simple method for direct observation and automated measurement of stomatal responses to bacterial invasion in Arabidopsis thaliana. This method leverages a portable stomatal imaging device, together with an image analysis pipeline designed for leaf images captured by the device.

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Chapters in this video

0:00

Introduction

1:10

Induction of Pseudomonas syringae pv. tomato DC3000 Infection in Arabidopsis thaliana for Automated Stomatal Response Measurement