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Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung

DOI :

10.3791/3857-v

7:46 min

June 5th, 2012

June 5th, 2012

31,524 Views

1Institute of Infection and Global Health, University of Liverpool , 2NIHR Biomedical Research Centre in Microbial Disease, University of Liverpool

Current diagnostic antimicrobial susceptibility testing relies on the planktonic growth of isolates in nutrient rich, aerobic conditions. Here, we employ an alternative artificial sputum medium to study antimicrobial susceptibility of Pseudomonas aeruginosa biofilms under both aerobic and microaerophilic conditions more representative of the cystic fibrosis lung.

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Artificial Sputum Medium

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