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Preparation of Single Cell Suspensions from Differentiated 3D Human Intestinal Organoids for Single Cell Transcriptomics

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02:22 min

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June 28th, 2024

DOI :

10.3791/201686-v

June 28th, 2024

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Carolyn Bomidi¹, Xi-Lei Zeng¹, Victoria Poplaski¹, Cristian Coarfa², Mary K. Estes¹, Sarah E. Blutt¹

¹Department of Molecular Virology and Microbiology, Baylor College of Medicine, ²Department of Molecular and Cellular Biology, Baylor College of Medicine

Transcript

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Single Cell Suspensions

Differentiated Organoids

Human Intestinal Organoids

Cell Recovery Solution

Enzymatic Cell Digestion

Cell Centrifugation

Trypan Blue Exclusion

Viable Cell Count

Differentiation Media

Absorptive Enterocytes

Secretory Cells

From the series

Integrating Single-Cell Transcriptomics with Human Intestinal Organoids

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