Evaluation of Mouse Retinal Pigmented Epithelium (RPE) by Light Microscopy
5:29
Evaluation of Mouse RPE Through Confocal Microscopy
6:32
Results: Investigating RPE Pathologies in Mice
8:35
Conclusion
Transcript
This protocol is the first to describe how to process and evaluate mouse size for RP pathologies using light transmission electron and confocal microscopy. The main advantage of this protocol is the inclusion of quantitative assessments of the RP
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Mouse models can be useful tools for investigating the biology of the retinal pigmented epithelium (RPE). It has been established that mice can develop an array of RPE pathologies. Here, we describe a phenotyping protocol to elucidate and quantify RPE pathologies in mice using light, transmission electron, and confocal microscopy.